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5 ARTICLES PUBLISHED IN JoVE

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Biology

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster
Justin B. Slawson 1, Eugene Z. Kim 1, Leslie C. Griffith 1
1National Center for Behavioral Genomics, Department of Biology, Volen Center for Complex Systems, Brandeis

The study of complex locomotor behavior in Drosophila melanogaster is dependent upon the ability to quantify changes in a given fly's movement. This article demonstrates how to do this using a high-resolution tracking system.

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Biology

Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions
Nicholas J. Hornstein 1, Stefan R. Pulver 1, Leslie C. Griffith 1
1Department of Biology, Brandeis

This procedure uses a blue light-activated algal channel and cell-specific genetic expression tools to evoke synaptic potentials with light pulses at the neuromuscular junction (NMJ) in Drosophila larvae. This technique is an inexpensive and easy-to-use alternative to suction electrode stimulation for synaptic physiology studies in research and teaching laboratories.

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Biology

Homarus Americanus Stomatogastric Nervous System Dissection
Anne-Elise Tobin 1, Hilary S. Bierman 1
1Volen Center for Complex Systems, Brandeis

We describe the fine dissection of the stomatogastric nervous system from the stomach of the American lobster (Homarus americanus).

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Biology

Cancer Borealis Stomatogastric Nervous System Dissection
Gabrielle J. Gutierrez 1, Rachel G. Grashow 1
1Volen Center for Complex Systems, Brandeis

The stomatogastric nervous system (STNS) of the Jonah crab (C. borealis) can be used for electrophysiology, immunohistochemistry, and cell culture studies. The STNS extraction is done in two parts: the gross and fine dissection.

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Biology

Gross Dissection of the Stomach of the Lobster, Homarus Americanus
Hilary S. Bierman 1, Anne-Elise Tobin 1
1Volen Center for Complex Systems, Brandeis

We describe the gross dissection of the stomach of the American lobster (Homarus americanus).

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