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Swedish University of Agricultural Sciences

5 ARTICLES PUBLISHED IN JoVE

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Biology

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
Anna Karlgren 1, Jenny Carlsson 2, Niclas Gyllenstrand 2, Ulf Lagercrantz 1, Jens F. Sundström 2
1Department of Evolutionary Functional Genomics, Evolutionary Biology Center, Uppsala University, 2Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences

We describe a modified DIG in situ hybridization protocol, which is fast and applicable on a wide range of plant species including Norway spruce. With just a few adjustments, including altered RNase treatment and proteinase K concentration, the protocol may be used in studies of different tissues and species.

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Biology

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
Pernilla Bjerling 1, Ida Olsson 1,2, Xi'nan Meng 1
1Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, University of Uppsala, 2Department of Microbiology, Swedish University of Agricultural Sciences

The fission yeast, Schizosaccharomyces pombe, is a good model system to study basic cellular processes. Here we describe a method to perform quantitative live cell analysis of fission yeast. In this particular experiment we focus on organisation of the genome within the cell nucleus, but the method can also be used to study cytosolic factors.

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Biology

Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival
Per Wessman 1, Sebastian Håkansson 1, Klaus Leifer 2, Stefano Rubino 2
1Department of Microbiology, Uppsala Biocenter, Swedish University of Agricultural Sciences, 2Department of Engineering Sciences, Uppsala University

Freeze-drying is often an easy and convenient way to obtain dry products of viable bacterial cells. An issue of the process is cell survival. We detail here a procedure to investigate how cell survival during freeze-drying is influenced by the properties of the formulation used.

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Biology

Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam
Stefano Rubino 1,4, Petter Melin 3, Paul Spellward 2, Klaus Leifer 1
1Department of Engineering Sciences, Uppsala University, 2Gatan Inc., 3Department of Microbiology, Swedish University of Agricultural Sciences, 4Physics Department, University of Oslo

Cryo Electron Microscopes, either Scanning (SEM) or Transmission (TEM), are widely used for characterization of biological samples or other materials with a high water content1. A SEM/Focused Ion Beam (FIB) is used to identify features of interest in samples and extract a thin, electron-transparent lamella for transfer to a cryo-TEM.

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Environment

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water
Lutz Ahrens 1, Atlasi Daneshvar 1,2, Anna E. Lau 1,2, Jenny Kreuger 1,2
1Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, 2Center for Chemical Pesticides, Swedish University of Agricultural Sciences

A protocol about the characterization and application of five different passive sampling devices is presented.

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