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University of Montreal

12 ARTICLES PUBLISHED IN JoVE

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Biology

Dissecting the Non-human Primate Brain in Stereotaxic Space
Mark W. Burke 1, Shahin Zangenehpour 2, Denis Boire 3, Maurice Ptito 2
1Department of Physiology, University of Montreal, 2School of Optometry, University of Montreal, 3Département de chimie-biologie
, Université du Québes à Trois-Rivières

The non-human primate is an important translational species for our understanding of the normal processing of the brain. The anatomical organization of the primate brain can provide important insights into normal and pathological conditions in humans.

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Biology

Brain Banking: Making the Most of your Research Specimens
Mark W. Burke 1, Shahin Zangenehpour 2, Maurice Ptito 2
1Department of Physiology, University of Montreal, 2School of Optometry, University of Montreal

Brain banking and systematic sampling of biological material provides the basis for unbiased stereology and maximizes the potential data obtained from each specimen.

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Biology

The Gateway to the Brain: Dissecting the Primate Eye
Mark Burke 1, Shahin Zangenehpour 2, Joseph Bouskila 2, Denis Boire 3, Maurice Ptito 2
1Department of Physiology, University of Montreal, 2School of Optometry, University of Montreal, 3Departement de chimie-biologie, Universite du Quebec a Trois-Rivieres

The non-human primate is an important translational species for our understanding of development and aging. The anatomical organization of the primate retina may provide important insights into normal and pathological conditions in humans.

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Biology

Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain
Mark Burke 1, Shahin Zangenehpour 2, Peter R. Mouton 3, Maurice Ptito 2
1Department of Physiology, University of Montreal, 2Ecole d’optometrie, University of Montreal, 3Stereology Resource Center

The anatomical organization of the primate brain can provide important insights into normal and pathological conditions in humans. Unbiased stereology is a method for accurately and efficiently estimating the total neuron number (or other cell type) in a given reference space1.

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Biology

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
Shahin Zangenehpour 1,2, Mark W. Burke 2, Avi Chaudhuri 3, Maurice Ptito 2
1Cognitive Neuroscience Unit, Montreal Neurological Institute, 2Ècole d’Optomètrie, Universitè de Montrèal, 3Department of Psychology, McGill University

Large-scale immunodetection of target proteins across the entire primate brain is possible by employing novel tissue embedding and sectioning methods combined with the use of creative apparatus for batch staining of multiple free-floating sections at a given time.

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Immunology and Infection

Murine Superficial Lymph Node Surgery
Mélissa Mathieu 1,2, Nathalie Labrecque 1,2,3
1Maisonneuve-Rosemont Hospital Research Center, 2Department of Microbiology and Immunology, University of Montreal, 3Department of Medicine, University of Montreal

To follow the progression of an immune response over time within the same mouse, lymph nodes can be sequentially removed by surgery. Here, we describe how this technique can be performed.

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Neuroscience

Progressive-ratio Responding for Palatable High-fat and High-sugar Food in Mice
Sandeep Sharma 1, Cecile Hryhorczuk 1, Stephanie Fulton 1
1CRCHUM and the Montreal Diabetes Research Center, University of Montreal

The present report details the protocol employed to measure the rewarding effects of high-fat food in mice using a progressive ratio operant conditioning task.

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Medicine

A Model of Chronic Nutrient Infusion in the Rat
Grace Fergusson 1, Mélanie Ethier 1, Bader Zarrouki 1,2, Ghislaine Fontés 1, Vincent Poitout 1,2
1Montreal Diabetes Research Center, CRCHUM, 2Department of Medicine, University of Montreal

A protocol for chronic infusions of glucose and Intralipid in rats is described. This model can be used to study the impact of nutrient excess on organ function and physiological parameters.

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Medicine

Chronic Thromboembolic Pulmonary Hypertension and Assessment of Right Ventricular Function in the Piglet
Pierre-Emmanuel Noly 1, Julien Guihaire 1,4, Matthieu Coblence 1, Peter Dorfmüller 2,5, Elie Fadel 1,3, Olaf Mercier 1,3
1Surgical Research Lab, Marie Lannelongue Hospital, 2Department of Pathology, Marie Lannelongue Hospital, 3Department of Thoracic and Vascular Surgery, Marie Lannelongue Hospital, 4Thoracic and Cardiovascular Surgery, University Hospital of Rennes, 5INSERM U999 Paris-Sud University

Chronic Thromboembolic Pulmonary Hypertension (CTEPH) and Right Ventricular (RV) dysfunction were induced in piglets by progressive obstruction of the pulmonary arteries. Consequences were remarkably similar to those observed in CTEPH patients. This animal model would be a very useful tool for pathophysiology and therapeutic experiments on CTEPH and RV failure.

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Genetics

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
Adjimon Gatien Lokossou 1, Chirine Toufaily 2, Amandine Vargas 3, Benoit Barbeau 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.

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Medicine

Assessment of Ex Vivo Murine Biventricular Function in a Langendorff Model
Pierre-Emmanuel Noly 1,2, Wei Huang 1, Suyash Naik 1, Paul Tang 1, Ienglam Lei 1
1University of Michigan, Ann Arbor, 2University of Montreal

Presented here is a protocol to reliably quantify the right and left ventricular function of donor hearts after cold preservation using an ex vivo perfusion system.

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Medicine

Endolymphatic Duct Blockage as a Surgical Treatment Option for Ménière's Disease
Annejet Alida Schenck 1,2, Issam Saliba 3, Josephina Maria Kruyt 4, Peter Paul van Benthem 2, Hendrikus Maria Blom 1,2,5
1Department of Otorhinolaryngology, Haga Hospital, 2Department of Otorhinolaryngology, Leiden University Medical Centre, 3Division of Otolaryngology-Head and Neck Surgery, Montreal University Hospital Center (CHUM), University of Montreal, 4Department of Otorhinolaryngology, Bergman Clinics, 5Department of Otorhinolaryngology, Antwerp University Hospital

Endolymphatic duct blockage is a relatively new surgical method for patients suffering from Ménière's disease. Following a regular mastoidectomy, the endolymphatic duct is identified and ligated using a regular titanium hemoclip. The effectiveness of this procedure is currently being assessed in a randomized trial.

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