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Donnelly Centre for Cellular and Biomolecular Research

1 ARTICLES PUBLISHED IN JoVE

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Biology

Digital Microfluidics for Automated Proteomic Processing
Mais J. Jebrail 1, Vivienne N. Luk 1,2, Steve C. C. Shih 2,3, Ryan Fobel 2,3, Alphonsus H. C. Ng 2,3, Hao Yang 1, Sergio L. S. Freire 1, Aaron R. Wheeler 1,2,3
1Department of Chemistry, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto

Digital Microfluidics is a technique characterized by the manipulation of discrete droplets (~nL - mL) on an array of electrodes by the application of electrical fields. It is well-suited for carrying out rapid, sequential, miniaturized automated biochemical assays. Here, we report a platform capable of automating several proteomic processing steps.

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