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University of Pittsburgh Medical Center

6 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Orthotopic Hind-Limb Transplantation in Rats
Robert Sucher *1, Rupert Oberhuber *1, Christian Margreiter 1, Guido Rumberg 1, Rishi Jindal 2, WP Andrew Lee 2, Raimund Margreiter 1, Johann Pratschke 1, Stefan Schneeberger 1, Gerald Brandacher 1
1Department of Visceral, Transplant, and Thoracic Surgery, Daniel Swarovski Research Laboratory, Innsbruck Medical University, 2Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Pittsburgh Medical Center

Here we describe the orthotopic rat hind-limb transplantation procedure, which seems to be the gold standard in vivo model for composite tissue allotransplantation research.

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Neuroscience

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
Michelle M. Sidor 1, Thomas J. Davidson 2, Kay M. Tye 3, Melissa R. Warden 4, Karl Diesseroth 2,5, Colleen A. McClung 1
1Department of Psychiatry, University of Pittsburgh Medical Center, 2Department of Bioengineering, Stanford University, 3Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory, Massachusetts Institute of Technology, 4Department of Neurobiology and Behavior, Cornell University, 5Department of Psychiatry and Behavioral Sciences, Stanford University

Optogenetics has become a powerful tool for use in behavioral neuroscience experiments. This protocol offers a step-by-step guide to the design and set-up of laser systems, and provides a full protocol for carrying out multiple and simultaneous in vivo optogenetic stimulations compatible with most rodent behavioral testing paradigms.

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Cancer Research

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
Phillip Huyett 1, Mark Gilbert 1, Lijun Liu 2, Robert L. Ferris 2, Seungwon Kim 1
1Department of Otolaryngology, University of Pittsburgh Medical Center, 2University of Pittsburgh Cancer Institute, Hillman Cancer Center

Perineural invasion (PNI) is a common feature of head and neck squamous cell carcinoma (HNSCC), conferring lower survival rates. Its mechanisms are poorly understood. Utilizing neurites generated from murine dorsal root ganglia confined to a semisolid matrix, the pathways involved in the PNI of HNSCC cell lines can be investigated.

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Immunology and Infection

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates
Alexander G. White *1, Pauline Maiello *1, M. Teresa Coleman 1, Jaime A. Tomko 1, L. James Frye 1, Charles A. Scanga 1, Philana Ling Lin 2, JoAnne L. Flynn 1
1Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, 2Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center

Here, we present a protocol to describe the analysis of 18F-FDG PET/CT imaging in non-human primates that have been infected with M. tuberculosis to study disease process, drug treatment, and disease reactivation.

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Behavior

Operative Technique and Nuances for the Stereoelectroencephalographic (SEEG) Methodology Utilizing a Robotic Stereotactic Guidance System
Amir H. Faraji 1, Zachary C. Gersey 2,3, Danielle M. Corson 2,3, James C. Sweat 2,3, Jorge A. Gonzalez-Martinez 2,3
1Department of Neurological Surgery, Houston Methodist, 2Department of Neurological Surgery, University of Pittsburgh Medical Center, 3Epilepsy Center, University of Pittsburgh Medical Center

The SEEG methodology is simplified and made faster with a stereotactic robot. Careful attention must be paid to the registration of the preoperative volumetric MRI to the patient prior to use of the robot in the OR. The robot streamlines the procedure, leading to decreased operative times and accurate implantations.

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Medicine

Technical Detail for Robot Assisted Pancreaticoduodenectomy
Alex C. Kim 1, Rebecca C. Rist 1, Amer H. Zureikat 1
1Department of Surgery, University of Pittsburgh Medical Center

The following manuscript details a stepwise approach to the robot-assisted pancreaticoduodenectomy performed at the University of Pittsburgh Medical Center.

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