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University of Bristol, UK

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging
Isabel Murillo 1, Mumtaz Virji 1
1Department of Cellular and Molecular Medicine, University of Bristol, UK

Neisseria meningitidis (Nm), a gram negative human-specific respiratory pathogen, can bind to human α-actinin. Here we present a protocol for visualisation of colocalisation of the bacterium with intracellular α-actinin after bacterial entry into human brain microvascular endothelial cells (HBMECs).

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