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2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
Michelle K. Leach 1, Zhang-Qi Feng 2, Caitlyn C. Gertz 3, Samuel J. Tuck 3, Tara M. Regan 3, Youssef Naim 3, Andrea M. Vincent 3, Joseph M. Corey 1,3,4
1Department of Biomedical Engineering, University of Michigan, 2State Key Laboratory of Bioelectronics, Southeast University, 3Department of Neurology, University of Michigan , 4Geriatric Research, Education and Clinical Center, Veterans Affairs Ann Arbor Health System

Aligned electrospun fibers direct the growth of neurons in vitro and are a potential component of nerve regeneration scaffolds. We describe a procedure for preparing electrospun fiber substrates and the serum-free culture of primary rat E15 sensory (DRG) and motor neurons. Visualization of neurons by immunocytochemistry is also included.

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Bioengineering

Electrospinning Fundamentals: Optimizing Solution and Apparatus Parameters
Michelle K. Leach 1, Zhang-Qi Feng 1,2, Samuel J. Tuck 3, Joseph M. Corey 1,3,4
1Department of Biomedical Engineering, University of Michigan, 2State Key Laboratory of Bioelectronics, Southeast University, 3Department of Neurology, University of Michigan, 4Geriatrics Research, Education and Clinical Center, Veterans Affairs Ann Arbor Healthcare Center

Electrospinning techniques can create a variety of nanofibrous scaffolds for tissue engineering or other applications. We describe here a procedure to optimize the parameters of the electrospinning solution and apparatus to obtain fibers with the desired morphology and alignment. Common problems and troubleshooting techniques are also presented.

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