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National Heart, Lung, and Blood Institute, National Institutes of Health

4 ARTICLES PUBLISHED IN JoVE

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Biology

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
Wenling Li 1, Yoh-suke Mukouyama 1
1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health

We introduce a whole-mount immunohistochemistry and laser scanning confocal microscopy with multiple labelling for analyzing intricate vascular network formation in mouse embryonic limb skin.

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Behavior

The Treadmill Fatigue Test: A Simple, High-throughput Assay of Fatigue-like Behavior for the Mouse
John P. Dougherty 1, Danielle A. Springer 2, Marvin C. Gershengorn 1
1Laboratory of Endocrinology and Receptor Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 2Murine Phenotyping Core, National Heart, Lung, and Blood Institute, National Institutes of Health

Fatigue is a common, undertreated and frequently poorly-understood symptom in many diseases and disorders. New preclinical assays of fatigue may help to improve current understanding and future treatment of fatigue. To that end, the current protocol provides a novel means of measuring fatigue-like behavior in the mouse.

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Cancer Research

A Mouse Model of Fatigue Induced by Peripheral Irradiation
Brian S. Wolff 1, Michael A. Renner 1, Danielle A. Springer 2, Leorey N. Saligan 1
1Symptom Biology Unit, National Institute of Nursing Research, National Institutes of Health, 2Murine Phenotyping Core, National Heart, Lung, and Blood Institute, National Institutes of Health

We describe a method using targeted peripheral irradiation to induce fatigue-like behavior in mice. The selected non-lethal irradiation dose leads to a week-long reduction in voluntary wheel-running activity.

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Developmental Biology

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
Tomoko Yamazaki 1,2, Wenling Li 1, Yoh-Suke Mukouyama 1
1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 2Earle A. Chiles Research Institute, Robert W. Franz Cancer Center, Providence Portland Medical Center

Here, we describe a high resolution whole-mount imaging method in the entire adult mouse ear skin, which enables us to visualize branching morphogenesis and patterning of peripheral nerves and blood vessels, as well as immune cell distribution.

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