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University of Oklahoma - Norman

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
Taylor R. Fore 1, Audrey A. Ojwang 1, Margaret L. Warner 1, Xinyun Peng 1, Rudolf A. Bohm 1,2, William P. Welch 1, Lindsey K. Goodnight 1, Hong Bao 1, Bing Zhang 1
1Department of Zoology, University of Oklahoma - Norman, 2Department of Biology, Brandeis University

We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.

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JoVE Core

Enhanced Electron Injection and Exciton Confinement for Pure Blue Quantum-Dot Light-Emitting Diodes by Introducing Partially Oxidized Aluminum Cathode
Zhibin Wang 1, Tai Cheng 1, Fuzhi Wang 1, Yiming Bai 1, Xingming Bian 1, Bing Zhang 1, Tasawar Hayat 2,3, Ahmed Alsaedi 3, Zhan'ao Tan 1
1State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, 2Department of Mathematics, Quaid-I-Azam University, 3NAAM Research Group, Faculty of Science, King Abdulaziz University

A protocol is presented for fabricating high-performance, pure blue ZnCdS/ZnS-based quantum dots light-emitting diodes by employing an autoxidized aluminum cathode.

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Biology

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells
Tianming Wang 1, Xu Chen 1, Ke Xu 1, Bing Zhang 1, Dexiang Huang 1, Jingwen Yang 1
1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University

This protocol provides an easy-to-handle method to culture the intestinal cells from sea cucumber Apostichopus japonicus and is compatible with a variety of widely available tissue samples from marine organisms including Echinodermata, Mollusca, and Crustacea.

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