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University of Wisconsin – Madison

3 ARTICLES PUBLISHED IN JoVE

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Biology

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow
Rebecca R. Vanderpool 1, Naomi C. Chesler 1
1Department of Biomedical Engineering, University of Wisconsin – Madison

The following protocol outlines the process of isolating, ventilating and instrumenting mouse lungs to measure steady or pulsatile pulmonary vascular pressure-flow relationships in order to quantify the effects of blood flow, airflow, airway changes and vascular changes on right ventricular afterload.

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Immunology and Infection

Glass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens
Hana T. Millen 1, Jordan C. Gonnering 1, Ryan K. Berg 2, Susan K. Spencer 3, William E. Jokela 3, John M. Pearce 4, Jackson S. Borchardt 1, Mark A. Borchardt 3
1Wisconsin Water Science Center, United States Geological Survey, 2University of Wisconsin – Madison, 3Agricultural Research Service, United States Department of Agriculture, 4Alaska Science Center, United States Geological Survey

Glass wool filters have been used to concentrate waterborne viruses by a number of research groups around the world. Here we show a simple approach for constructing glass wool filters and demonstrate the filters are also effective in concentrating waterborne viral, bacterial and protozoan pathogens.

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Biology

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
Jinfeng Teng 1, Stephen Loukin 1, Xinliang Zhou 1, Ching Kung 1,2
1Laboratory of Cell and Molecular Biology, University of Wisconsin – Madison, 2Department of Genetics, University of Wisconsin – Madison

To complement commonly used methods to study TRPV4’s, two methods are described: Its mechanosensitivity can be studied by Ca2+-aequorin luminometry in transgenic yeast upon hypo-osmotic challenge. It can also be examined in TRPV4-RNA injected Xenopus oocytes by whole-cell two-electrode voltage clamp or patch clamp in on-cell or excised mode.

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