University of Wisconsin – Madison

3 ARTICLES PUBLISHED IN JoVE

Biology

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow

Rebecca R. Vanderpool¹, Naomi C. Chesler¹

¹Department of Biomedical Engineering, University of Wisconsin – Madison

The following protocol outlines the process of isolating, ventilating and instrumenting mouse lungs to measure steady or pulsatile pulmonary vascular pressure-flow relationships in order to quantify the effects of blood flow, airflow, airway changes and vascular changes on right ventricular afterload.

Immunology and Infection

Glass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens

Hana T. Millen¹, Jordan C. Gonnering¹, Ryan K. Berg², Susan K. Spencer³, William E. Jokela³, John M. Pearce⁴, Jackson S. Borchardt¹, Mark A. Borchardt³

¹Wisconsin Water Science Center, United States Geological Survey, ²University of Wisconsin – Madison, ³Agricultural Research Service, United States Department of Agriculture, ⁴Alaska Science Center, United States Geological Survey

Glass wool filters have been used to concentrate waterborne viruses by a number of research groups around the world. Here we show a simple approach for constructing glass wool filters and demonstrate the filters are also effective in concentrating waterborne viral, bacterial and protozoan pathogens.

Biology

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Jinfeng Teng¹, Stephen Loukin¹, Xinliang Zhou¹, Ching Kung^1,2

¹Laboratory of Cell and Molecular Biology, University of Wisconsin – Madison, ²Department of Genetics, University of Wisconsin – Madison

To complement commonly used methods to study TRPV4’s, two methods are described: Its mechanosensitivity can be studied by Ca²⁺-aequorin luminometry in transgenic yeast upon hypo-osmotic challenge. It can also be examined in TRPV4-RNA injected Xenopus oocytes by whole-cell two-electrode voltage clamp or patch clamp in on-cell or excised mode.