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9 ARTICLES PUBLISHED IN JoVE

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Biology

Fabrication of the Thermoplastic Microfluidic Channels
Arpita Bhattacharyya 1, Dominika Kulinski 1, Catherine Klapperich 1
1Department of Biomedical Engineering, Boston University

Here we demonstrate how to fabricate thermoplastic microfluidic chips using hot embossing and heat sealing. Then we demonstrate how to use in situ light directed surface grafting and polymerization through the sealed chip to form the composite solid phase columns.

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Biology

Microfluidic Applications for Disposable Diagnostics
Catherine Klapperich 1
1Department of Biomedical Engineering, Boston University

In this interview, Dr. Klapperich discusses the fabrication of thermoplastic microfluidic devices and their application for development of new diagnostics.

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Bioengineering

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
Carlos A. Lopez 1, George G. Daaboul 2, Sunmin Ahn 2, Alexander P. Reddington 1, Margo R. Monroe 2, Xirui Zhang 2, Rostem J. Irani 3, Chunxiao Yu 4,5, Caroline A. Genco 4,5, Marina Cretich 6, Marcella Chiari 6, Bennett B. Goldberg 1, John H. Connor 5, M. Selim Ünlü 1,2
1Department of Electrical and Computer Engineering, Boston University , 2Department of Biomedical Engineering, Boston University , 3Center for Advanced Genomics Technology, Boston University , 4Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, 5Department of Microbiology, Boston University School of Medicine, 6CNR (National Research Council), Istituto di Chimica del Riconoscimento Molecolare

Quantitative, high-throughput, real-time, and label-free biomolecular detection (DNA, protein, etc.) on SiO2 surfaces can be achieved using a simple interferometric technique which relies on LED illumination, minimal optical components, and a camera. The Interferometric Reflectance Imaging Sensor (IRIS) is inexpensive, simple to use, and amenable to microarray formats.

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Engineering

Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation
Jonathan A. Kopechek 1, Peng Zhang 1, Mark T. Burgess 1, Tyrone M. Porter 1
1Department of Mechanical Engineering, Boston University

Phase-shift nanoemulsions (PSNE) can be vaporized using high intensity focused ultrasound to enhance localized heating and improve thermal ablation in tumors. In this report, the preparation of stable PSNE with a narrow size distribution is described. Furthermore, the impact of vaporized PSNE on ultrasound-mediated ablation is demonstrated in tissue-mimicking phantoms.

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Bioengineering

Microfluidic Chip Fabrication and Method to Detect Influenza
Qingqing Cao 1, Andy Fan 2, Catherine Klapperich 1,2
1Department of Mechanical Engineering, Boston University , 2Department of Biomedical Engineering, Boston University

An integrated microfluidic thermoplastic chip has been developed for use as a molecular diagnostic. The chip performs nucleic acid extraction, reverse transcriptase, and PCR. Methods for fabricating and running the chip are described.

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Biology

Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis
Shinichiro Yokota 1,2, Yoshihiro Ono 2, Toshimasa Nakao 2, Peng Zhang 3, George K. Michalopoulos 4,5, Zahida Khan 3,4,5,6
1Department of Surgery, Jichi Medical University, 2Department of Surgery, University of Pittsburgh School of Medicine, 3Department of Pediatrics, University of Pittsburgh School of Medicine, 4Department of Pathology, University of Pittsburgh School of Medicine, 5Pittsburgh Liver Research Center, University of Pittsburgh, 6McGowan Institute for Regenerative Medicine, University of Pittsburgh

Here, we present partial bile duct ligation as a surgical model of liver injury and regeneration in rodents.

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Genetics

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Jinxia Shi 1, Yijuan Jia 1, Di Fang 1, Shidan He 1, Peng Zhang 1,2, Yushuang Guo 3, Yongli Qiao 1
1Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, 2College of Agriculture, Yangtze University, 3Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou Institute of Tobacco Science

Here, we present a modified screening method that can be extensively used to quickly screen RNA silencing suppressors in plant pathogens.

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Bioengineering

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells
Connor S. Centner 1, Emily M. Murphy 1, Bryce F. Stamp 2, Mariah C. Priddy 1, John T. Moore 1, Paula J. Bates 2, Michael A. Menze 3, Kavitha Yaddanapudi 2, Jonathan A. Kopechek 1
1Department of Bioengineering, University of Louisville, 2School of Medicine, University of Louisville, 3Department of Biology, University of Louisville

This protocol describes the assembly and operation of a low-cost acoustofluidic device for rapid molecular delivery to cells via sonoporation induced by ultrasound contrast agents.

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JoVE Core

A Modeling and Simulation Method for Preliminary Design of an Electro-Variable Displacement Pump
Xu Han 1, Peng Zhang 2, Tatiana Minav 3, Yongling Fu 1, Jian Fu 1
1School of Mechanical Engineering and Automation, Beihang University, 2Beijing Institute of Precision Mechatronics and Controls, 3Innovative Hydraulics and Automation, Tampere University

A simulation model specifically supporting the preliminary design of an electro-variable displacement pump (EVDP) is developed and partially verified by experiments. The control performance, life, reliability, etc., can all be evaluated using the proposed model, which covers the main performance requirements under the EVDP preliminary design task.

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