University of North Carolina

2 ARTICLES PUBLISHED IN JoVE

Biology

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Adam J. Kole¹, Elizabeth R.W. Knight², Mohanish Deshmukh¹

¹Department of Cell and Developmental Biology, Neuroscience Center, University of North Carolina , ²Curriculum in Neurobiology, Neuroscience Center, University of North Carolina

In this protocol, we describe the direct cytoplasmic microinjection of cytochrome c protein into fibroblasts and primary sympathetic neurons. This technique allows for the introduction of cytochrome c protein into the cytoplasm of cells and mimics the release of cytochrome c from mitochondria, which occurs during apoptosis.

Bioengineering

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

Shrirang Karve^1,2, Michael E. Werner^1,2, Natalie D. Cummings^1,2, Rohit Sukumar^1,2, Edina C. Wang^1,2, Ying-Ao Zhang^1,2, Andrew Z. Wang^1,2

¹Laboratory of Nano- and Translational Medicine, Department of Radiation Oncology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, ²Carolina Center for Nanotechnology Excellence, University of North Carolina

This article describes a nanoprecipitation method to synthesize polymer-based nanoparticles using diblock co-polymers. We will discuss the synthesis of diblock co-polymers, the nanoprecipitation technique, and potential applications.