Wayne State University

2 ARTICLES PUBLISHED IN JoVE

Biology

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Derek J. Averill^*1, Joel Garcia^*1, Buddhima N. Siriwardena-Mahanama^*1, Sashiprabha M. Vithanarachchi^*1, Matthew J. Allen¹

¹Department of Chemistry, Wayne State University

We demonstrate the metalation, purification, and characterization of lanthanide complexes. The complexes described here can be conjugated to macromolecules to enable tracking of these molecules using magnetic resonance imaging.

Medicine

MAME Models for 4D Live-cell Imaging of Tumor: Microenvironment Interactions that Impact Malignant Progression

Mansoureh Sameni¹, Arulselvi Anbalagan¹, Mary B. Olive¹, Kamiar Moin^1,2, Raymond R. Mattingly^1,2, Bonnie F. Sloane^1,2

¹Department of Pharmacology, Wayne State University , ²Barbara Ann Karmanos Cancer Institute, Wayne State University

We have developed 3D coculture models for live-cell imaging in real-time of interactions among breast tumor cells and other cells in their microenvironment that impact progression to an invasive phenotype. These models can serve as preclinical screens for drugs to target paracrine-induced proteolytic, chemokine/cytokine and kinase pathways implicated in invasiveness.