The Ohio State University Medical Center

9 ARTICLES PUBLISHED IN JoVE

Biology

Jiao-Jing Wang¹, Sara Hockenheimer², Alice A. Bickerstaff¹, Gregg A. Hadley¹

¹Department of Surgery, The Ohio State University, ²Integrated Biomedical Sciences Graduate Program, The Ohio State University

Renal transplantation in mice is a technically challenging procedure that requires careful post-operative care and treatment for success.

Medicine

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart

Surya C. Gnyawali^1,2, Sashwati Roy^1,2, Jason Driggs^1,2, Savita Khanna^1,2, Thomas Ryan^2,3, Chandan K. Sen²

¹Department of Surgery, The Ohio State University, ²Heart and Lung Research Institute, The Ohio State University, ³Department of Cardiovascular Medicine, The Ohio State University

High frequency Doppler ultrasound is a novel technology for assessing regional myocardial function. This work presents first evidence demonstrating applicability of this versatile imaging platform for the repeated measure of myocardial strain, dp/dt, and mitral regurgitation in the ischemia-reperfused (IR) murine heart.

Medicine

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function

Ronald X. Xu¹, Kun Huang², Ruogu Qin¹, Jiwei Huang¹, Jeff S. Xu¹, Liya Ding², Urmila S. Gnyawali³, Gayle M. Gordillo³, Surya C. Gnyawali^3,4, Chandan K. Sen³

¹Department of Biomedical Engineering, The Ohio State University, ²Department of Biomedical Informatics, The Ohio State University, ³Comprehensive Wound Center, The Ohio State University, ⁴Department of Surgery, The Ohio State University

A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.

Medicine

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens

Kaushal Joshi¹, Habibe Demir¹, Ryosuke Yamada¹, Takeshi Miyazaki¹, Abhik Ray-Chaudhury², Ichiro Nakano¹

¹Department of Neurological Surgery, The Ohio State University Medical Center, ²Department of Pathology, The Ohio State University Medical Center

Here, we established a method for drug efficacy testing with surgical specimens of brain tumors, termed “tumor explant method”. With this method, we can evaluate drug efficacy without breaking the microenvironment of solid tumors. To validate reliability of this method, we describe representative data with our glioma specimen treated with the current first-line chemotherapeutic agent, temozolomide.

Immunology and Infection

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Bryan A. Anthony¹, Gregg A. Hadley¹

¹Department of Surgery, The Ohio State University Medical Center

Murine bone marrow transplantation is a widely used technique to study immunological mechanisms governing graft-versus-host disease in humans. The ability to monitor T cell trafficking patterns in vivo allows for detailed analysis of the development and perpetuation of T cell responses during graft-versus-host disease.

Medicine

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

Zhengtao Chu^1,2, Kathleen LaSance³, Victor Blanco¹, Chang-Hyuk Kwon^5,6, Balveen Kaur^5,6, Malinda Frederick⁴, Sherry Thornton⁴, Lisa Lemen³, Xiaoyang Qi^1,2

¹Division of Hematology-Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine, ²Division of Human Genetics, University of Cincinnati College of Medicine, ³Department of Radiology, University of Cincinnati College of Medicine, ⁴Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, ⁵Solid Tumor Biology Program, James Comprehensive Cancer Center, The Ohio State University Medical Center, ⁶Department of Neurological Surgery, James Comprehensive Cancer Center, The Ohio State University Medical Center

We describe a multi-angle rotational optical imaging (MAROI) system for in vivo quantitation of a fluorescent marker delivered by saposin C (SapC)-dioleoylphosphatidylserine (DOPS) nanovesicles. Employing mouse models of cancer and arthritis, we demonstrate how the MAROI signal curve analysis can be used for the precise mapping and biological characterization of disease processes.

Medicine

Ferric Chloride-induced Canine Carotid Artery Thrombosis: A Large Animal Model of Vascular Injury

Allyson L. Huttinger¹, Debra G. Wheeler¹, Surya Gnyawali², David Dornbos III¹, Juliana M. Layzer³, Nicholas Venetos¹, Spencer Talentino¹, Nicholas J. Musgrave¹, Cheyenne Jones¹, Camille Bratton¹, Matthew E. Joseph⁴, Chandan Sen², Bruce A. Sullenger³, Shahid M. Nimjee¹

¹Department of Neurological Surgery, Ohio State University, ²Department of Surgery, Ohio State University, ³Department of Surgery, Duke University, ⁴Department of Internal Medicine, Ohio State University

Here, we present the modifications necessary to a well characterized and commonly used small animal ferric chloride-induced (FeCl₃) carotid artery injury model for use in a large animal vascular injury model. The resulting model can be utilized for pre-clinical trial assessment of both prophylactic and thrombolytic pharmacological and mechanical interventions.

Cancer Research

Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells

Katie A Thies¹, Sarah Steck¹, Sue E Knoblaugh², Steven T Sizemore¹

¹Department of Radiation Oncology, Arthur G. James Comprehensive Cancer Center and Richard L. Solove Research Institute, The Ohio State University Medical Center, ²Department of Veterinary Biosciences, Comparative Pathology and Digital Imaging Shared Resource, The Ohio State University

Intravenous injection of cancer cells is often used in metastasis research, but the metastatic tumor burden can be difficult to analyze. Herein, we demonstrate a tail-vein injection model of metastasis and include a novel approach to analyze the resulting metastatic lung tumor burden.

Neuroscience

Quantification of Cerebral Perfusion using Laser Speckle Imaging and Infarct Volume using MRI in a Pre-clinical Model of Posterior Circulation Stroke

Surya C. Gnyawali¹, Debra G. Wheeler¹, Allyson L. Huttinger¹, Cole Anderson¹, Ian Mandybur¹, Catherine Lee¹, Caitlin Hatten¹, Jenna Boue², Matthew Joseph³, Shahid M. Nimjee^1,2,4

¹Department of Neurological Surgery, The Ohio State University Medical Center, ²Department of Anatomy, The Ohio State University Medical Center, ³Department of Internal Medicine, The Ohio State University Medical Center, ⁴Department of Radiology, The Ohio State University Medical Center

A canine model of LVO stroke was utilized to develop laser speckle imaging to monitor cerebral perfusion in real-time. Diffusion-weighted MRI was optimized to image infarct volume utilizing a high b-value, enabling ADC and MRA, correlated with DSA at the time of stroke. Finally, ADC reconstructions correlated with histological findings.