Robert Koch-Institute

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

Diana Pauly¹, Pablo A. Chacana², Esteban G. Calzado³, Björn Brembs⁴, Rüdiger Schade⁵

¹Center for Biological Security, Robert Koch-Institute, ²CICVyA - INTA Castelar, Instituto de Virología, ³Center of Molecular Immunology, Ciudad de la Habana, Cuba, ⁴Department of Biology, Chemistry, Pharmacy, Institute of Biology-Neurobiology, Free University of Berlin, ⁵Institut of Pharmacology, Charité-University Medicine of Berlin

This protocol describes in particular the extraction of total IgY from egg yolk by means of polyethylene glycol precipitation and gives general information about IgY technology.

Biology

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Eloïse Mussard^1,2, Corinne Lencina¹, Gaëlle Boudry³, Caroline S. Achard², Christian Klotz⁴, Sylvie Combes¹, Martin Beaumont¹

¹GenPhySE, Université de Toulouse, INRAE, ENVT, ²Lallemand Animal Nutrition, ³Institut NuMeCan, INRAE, INSERM, Université de Rennes, ⁴FG 16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute

Here, we describe a protocol to culture pig intestinal 3D organoids from cryopreserved epithelial crypts. We also describe a method to establish cell monolayers derived from 3D organoids, allowing access to the apical side of epithelial cells.