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System Biosciences

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles
Amy Mendenhall 1, Jacob Lesnik 1, Chandreyee Mukherjee 1, Travis Antes 1, Ranjita Sengupta 1
1System Biosciences

Lentiviral expression vectors are the most effective vehicles for stably expressing different effector molecules or reporter constructs in dividing and non-dividing mammalian cells and whole organisms. Here we provide a protocol on how to package lentivector expression constructs in pseudoviral particles and to transduce target cells using the pseudoviral particles.

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Education

CRISPR-Mediated Reorganization of Chromatin Loop Structure
Stefanie L. Morgan *1,2, Erin Y. Chang *1, Natasha C. Mariano 1, Abel Bermudez 3, Nicole L. Arruda 4, Fanting Wu 5, Yunhai Luo 1, Gautam Shankar 1, Star K. Huynh 1, Chiao-Chain Huang 5, Sharon J. Pitteri 3, Kevin C. Wang 1,2,6
1Department of Dermatology, Program in Epithelial Biology, Stanford University School of Medicine, 2Program in Cancer Biology, Stanford University School of Medicine, 3Canary Center for Cancer Early Detection, Department of Radiology, Stanford University School of Medicine, 4Department of Biology, Bridgewater State University, 5System Biosciences, 6Veterans Affairs Healthcare System

Chromatin looping plays a significant role in gene regulation; however, there have been no technological advances that allow for selective and reversible modification of chromatin loops. Here we describe a powerful system for chromatin loop re-organization using CRISPR-dCas9 (CLOuD9), demonstrated to selectively and reversibly modulate gene expression at targeted loci.

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