University of Texas Southwestern Medical Center

5 ARTICLES PUBLISHED IN JoVE

Medicine

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model

Debabrata Saha¹, Henry Dunn², Heling Zhou², Hiroshi Harada³, Masahiro Hiraoka³, Ralph P. Mason², Dawen Zhao²

¹Department of Radiation Oncology, University of Texas Southwestern Medical Center , ²Department of Radiology, University of Texas Southwestern Medical Center , ³Department of Radiation Oncology, Kyoto University Graduate School of Medicine

Bioluminescence imaging of hypoxia inducible factor-1α activity is applied to monitor intracranial tumor hypoxia development in a breast cancer brain metastasis mouse model.

Immunology and Infection

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract

Eduardo Lopez-Medina¹, Megan M. Neubauer¹, Gerald B. Pier², Andrew Y. Koh³

¹Department of Pediatrics, University of Texas Southwestern Medical Center , ²Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, ³Department of Pediatrics and Microbiology, University of Texas Southwestern Medical Center

A reliable method for the RNA isolation of Pseudomonas aeruginosa recovered from murine cecums is described. The RNA recovered is of sufficient quantity and quality for subsequent qPCR, transcription profiling, and RNA Seq experiments. This technique can be adapted for RNA isolation of other intestinal microbes.

Biology

Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

L. Evan Reddick¹, Neal M. Alto¹

¹Department of Molecular Microbiology, University of Texas Southwestern Medical Center

The CLEM technique has been adapted to analyze ultrastructural morphology of membranes, organelles, and subcellular structures affected by microinjected molecules. This method combines the powerful techniques of micromanipulation/microinjection, confocal fluorescent microscopy, and electron microscopy to allow millimeter to multi-nanometer resolution. This technique is amenable to a wide variety of applications.

JoVE Core

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering

Bahar Bilgen^1,2, Danielle Chu³, Robert Stefani¹, Roy K. Aaron^1,2

¹Department of Orthopaedics, The Warren Alpert Brown Medical School of Brown University and the Rhode Island Hospital, ²Center for Restorative and Regenerative Medicine, VA Medical Center, Providence, RI, ³University of Texas Southwestern Medical Center

We designed a novel mechanical loading bioreactor that can apply uniaxial or biaxial mechanical strain to a cartilage biocomposite prior to transplantation into an articular cartilage defect.

Medicine

Ultrasound Imaging-guided Intracardiac Injection to Develop a Mouse Model of Breast Cancer Brain Metastases Followed by Longitudinal MRI

Heling Zhou¹, Dawen Zhao¹

¹Radiology, University of Texas Southwestern Medical Center

A breast cancer brain metastasis mouse model is established with ultrasound imaging-guided intracardiac injection of MDA-MB231/Br-GFP cells. Development of multifocal intracranial metastases has been monitored longitudinally using high-resolution 9.4 T MRI.