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National Research Council of Canada

5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
Christophe Py 1, Marzia Martina 2, Robert Monette 2, Tanya Comas 2, Mike W. Denhoff 1, Collin Luk 3, Naweed I. Syed 3, Geoff Mealing 2
1Institute for Microstructural Sciences, National Research Council of Canada, 2Institute for Biological Sciences, National Research Council of Canada, 3Hotchkiss Brain Institute, University of Calgary

We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.

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Immunology and Infection

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Tim J. Dumonceaux 1, Jennifer R. Town 1, Janet E. Hill 2, Bonnie L. Chaban 2, Sean M. Hemmingsen 3
1Saskatoon Research Centre, Agriculture and Agri-Food Canada, 2Department of Veterinary Microbiology, University of Saskatchewan , 3Plant Biotechnology Institute, National Research Council of Canada

We describe a multiplex method for the detection of microorganisms within a sample using oligonucleotide-coupled fluorescent beads. Amplicon from all organisms within a sample is hybridized to a panel of probe-coupled beads. A Luminex or Bio-Plex instrument is used to query each bead for bead type and hybridization signal.

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Engineering

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Robert F. Peters 1,2, Luis Gutierrez-Rivera 1,2, Steven K. Dew 1,2, Maria Stepanova 1,2
1Department of Electrical and Computer Engineering, University of Alberta, 2National Institute for Nanotechnology, National Research Council of Canada

We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.

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Bioengineering

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
Lidija Malic 1, Abdelrahman Elmanzalawy 1, Jamal Daoud 2, Matthias Geissler 1, Alex Boutin 1, Ljuboje Lukic 1, Mojra Janta 1, Dillon Da Fonte 1, Christina Nassif 1, Teodor Veres 1
1Life Sciences Division, National Research Council of Canada, 2Galenvs Sciences, Inc.

Epigenetic markers are used for white blood cell (WBC) subtyping through the quantification of DNA methylation patterns. This protocol presents a multiplex droplet polymerase chain reaction (mdPCR) method using a thermoplastic elastomer (TPE)-based microfluidic device for droplet generation allowing for precise and multiplex methylation-specific target quantification of WBC differential counts.

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Cancer Research

A Dorsal Skinfold Window Chamber Tumor Mouse Model for Combined Intravital Microscopy and Magnetic Resonance Imaging in Translational Cancer Research
W. Jeffrey Zabel *1, Nader Allam *1, Hector Alejandro Contreras Sanchez 1, Warren Foltz 2,3, Costel Flueraru 4, Edward Taylor 2,3, Alex Vitkin 1,2,3
1Department of Medical Biophysics, University of Toronto, 2Radiation Medicine Program, Princess Margaret Cancer Centre, 3Department of Radiation Oncology, University of Toronto, 4Advanced Electronic and Photonics Research Center, National Research Council of Canada

Translation of Intravital microscopy findings is challenged by its shallow depth penetration into tissue. Here we describe a dorsal window chamber mouse model that enables co-registration of intravital microscopy and clinically applicable imaging modalities (e.g., CT, MRI) for direct spatial correlation, potentially streamlining clinical translation of intravital microscopy findings.

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