Texas A&M University System Health Science Center, College of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Visualization of Caenorhabditis elegans Cuticular Structures Using the Lipophilic Vital Dye DiI

¹Department of Molecular and Cellular Medicine, Texas A&M University System Health Science Center, College of Medicine

We present a method to visualize cuticle in live C. elegans using the red fluorescent lipophilic dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), which is commonly used in C. elegans to visualize environmentally exposed neurons. With this optimized protocol, alae and annular cuticular structures are stained by DiI and observed using compound microscopy.

Biology

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Katherine K. Beifuss¹, Tina L. Gumienny¹

¹Department of Molecular and Cellular Medicine, Texas A&M University System Health Science Center

We describe a sensitized method to identify postembryonic regulators of protein expression and localization in C. elegans using an RNAi-based genomic screen and an integrated transgene that expresses a functional, fluorescently tagged protein.