Technopôle de Château-Gombert

2 ARTICLES PUBLISHED IN JoVE

Biology

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Vincent Rouger^1,2,3, Nicolas Bertaux^4,5,6, Tomasz Trombik^1,2,3, Sébastien Mailfert^1,2,3, Cyrille Billaudeau^1,2,3, Didier Marguet^1,2,3, Arnauld Sergé^1,2,3

¹Institut National de la Santé et de la Recherche Médicale, UMR 631, Parc scientifique de Luminy, ²Centre National de la Recherche Scientifique, UMR 6102, Parc scientifique de Luminy, ³Centre d'Immunologie de Marseille-Luminy, Aix-Marseille University, ⁴École Centrale Marseille, Technopôle de Château-Gombert, ⁵Institut Fresnel, Aix-Marseille University, ⁶Centre National de la Recherche Scientifique, UMR 6133, Aix-Marseille University

Multiple-Target Tracing is a homemade algorithm developed for tracking individually labeled molecules within the plasma membrane of living cells. Efficiently detecting, estimating and tracing molecules over time at high-density provide a user-friendly, comprehensive tool to investigate nanoscale membrane dynamics.

Biochemistry

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Sebastien Mailfert^*1, Karolina Wojtowicz^*2, Sophie Brustlein^*1, Ewa Blaszczak³, Nicolas Bertaux⁴, Marcin Łukaszewicz², Didier Marguet¹, Tomasz Trombik²

¹CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, ²Faculty of Biotechnology, University of Wroclaw, ³Faculty of Biological Sciences, University of Wroclaw, ⁴CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ

This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.