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Weill Cornell Medical College

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Silk Film Culture System for in vitro Analysis and Biomaterial Design
Brian D. Lawrence 1, Zhi Pan 1, Michael D. Weber 1, David L. Kaplan 2, Mark I. Rosenblatt 1
1Margaret M. Dyson Vision Research Institute, Weill Cornell Medical College , 2Department of Biomedical Engineering, Tufts University

Silk films are a novel class of biomaterials readily customizable for an array of biomedical applications. The presented silk film culture system is highly adaptable to a variety of in vitro analyses. This system represents a biomaterial design platform offering in vitro optimization before direct translation to in vivo models.

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Medicine

The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva
Cynthia L. Bristow 1, Mariya A. Babayeva 1, Rozbeh Modarresi 1, Carole P. McArthur 2, Santosh Kumar 3, Charles Awasom 4, Leo Ayuk 4, Annette Njinda 5, Paul Achu 5, Ronald Winston 6
1Department of Medicine, Weill Cornell Medical College , 2Department of Oral Biology, University of Missouri-Kansas City-School of Dentistry, 3Department of Pharmacology and Toxicology, University of Missouri Kansas City- School of Pharmacy, 4Regional Hospital, Bamenda, NWP, Cameroon, 5Mezam Polyclinic HIV/AIDS Treatment Center, Cameroon, 6Institute for Human Genetics and Biochemistry

A CD4 enumeration method, the α-test, is described which uses whole saliva to provide rapid and accurate CD4 counts. The α-test costs pennies and eliminates the need for technical training, costly reagents such as monoclonal antibodies, instrumentation, refrigeration, transport of samples, as well as collection and handling of blood.

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Biology

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines
Laura A. Martin 1, Marco Seandel 1
1Department of Surgery, Weill Cornell Medical College

A simple method to derive and maintain spermatogonial stem and progenitor cell lines from adult mice is presented here. The method utilizes feeder cells originating from the somatic cell compartment of the adult mouse testis. This technique is applicable to common mouse strains, including transgenic, knock-out, and knock-in mice.

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Medicine

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
Sharanya Iyengar 1, Yariv Houvras 2,3, Craig J. Ceol 1
1Program in Molecular Medicine and Department of Cancer Biology, University of Massachusetts Medical School, 2Departments of Surgery and Medicine, Weill Cornell Medical College , 3Departments of Surgery and Medicine, New York Presbyterian Hospital

A rapid way to screen for melanoma modifiers using a zebrafish autochthonous tumor model is presented. It takes advantage of the miniCoopR vector which allows for expression of candidate melanoma genes in melanocytes. A method to obtain melanoma-free survival curves, an invasion assay, a protocol for antibody staining of scale melanocytes and a melanoma transplantation assay are described.

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Behavior

Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
Kathryn C. Schierberl 1,2, Anjali M. Rajadhyaksha 1,2
1Graduate Program in Neuroscience, Weill Cornell Graduate School of Biomedical Sciences, 2Division of Pediatric Neurology, Department of Pediatrics, Weill Cornell Medical College

This article describes how to microinject viral vectors into mouse brain and then test in a conditioned place preference paradigm that includes an acquisition, extinction and reinstatement phase.

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