Universitá degli Studi di Milano

2 ARTICLES PUBLISHED IN JoVE

Biology

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Michela Frascoli¹, Michele Proietti¹, Fabio Grassi^1,2

¹Institute for Research in Biomedicine, Bellinzona (Switzerland), ²Dipartimento di Biologia e Genetica per le Scienze Mediche, Universitá degli Studi di Milano

A method to analyse the distribution of bone marrow hematopoietic progenitors in flow cytometry as well as to efficiently isolate highly purified hematopoietic stem cells (HSCs) is described. The isolation procedure is essentially based on magnetic enrichment of c-Kit+ cells and cell sorting to purify HSCs for cellular and molecular studies.

Developmental Biology

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

Mário Costa^1,2, Ana Marta Pereira³, Sílvia Coimbra^1,2

¹Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, ²Laboratório Associado para a Química Verde, ³Dipartimento di Bioscienze, Universitá degli Studi di Milano

This protocol describes in detail how the plant material for immunolocalization of Arabinogalactan proteins and pectins is fixed, embedded in a hydrophilic acrylic resin, sectioned and mounted on glass slides. We show cell wall related epitopes will be detected with specific antibodies.