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Southern Illinois University

5 ARTICLES PUBLISHED IN JoVE

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Biology

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Navneet Dogra 1, Julia C. Reyes 1, Nishi Garg 1, Punit Kohli 1
1Department of Chemistry and Biochemistry, Southern Illinois University

We demonstrate FRET between conjugated polymer polydiacetylene (PDA) and fluorophore attached to the surface of PDA liposomes for the sensing of biomolecules. PDA liposomes also contained receptor molecules on their surfaces for biomolecules to be used as probes. Ligand-receptor interactions lead to changes in the FRET efficiency between the fluorophore and PDA which is the basis of the sensing mechanism.

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Environment

A Method for Quantifying Foliage-Dwelling Arthropods
Michael W. Eichholz 1, Kevin P. Sierzega 1
1Cooperative Wildlife Research Laboratory, Center for Ecology, Department of Zoology, Southern Illinois University

We describe how to quantify leaf dwelling arthropods by sealing the leaves and end of branches in a bag, clipping and freezing the bagged material, and rinsing the previously frozen material in water to separate arthropods from the substrate for quantification.

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Environment

Quantifying Corticolous Arthropods Using Sticky Traps
Michael W. Eichholz *1, Elise C. Zarri *2, Kevin P. Sierzega *1
1Cooperative Wildlife Research Laboratory, Center for Ecology, Department of Zoology, Southern Illinois University, 2Montana Cooperative Research Unit, University of Montana

We describe a semi-quantitative approach of measuring characteristics of corticolous (bark-dwelling) arthropod communities. We placed commercially manufactured sticky traps on tree boles to estimate abundance, total length (a surrogate to biomass), richness, and Shannon diversity for comparison among tree species.

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Environment

Simulating Impacts of Ice Storms on Forest Ecosystems
John L. Campbell 1, Lindsey E. Rustad 1, Charles T. Driscoll 2, Ian Halm 3, Timothy J. Fahey 4, Habibollah Fakhraei 5, Peter M. Groffman 6,7, Gary J. Hawley 8, Wendy Leuenberger 9, Paul G. Schaberg 10
1Northern Research Station, U.S. Forest Service, Durham, NH, 2Department of Civil and Environmental Engineering, Syracuse University, 3Northern Research Station, U.S. Forest Service, North Woodstock, NH, 4Department of Natural Resources, Cornell University, 5Department of Civil and Environmental Engineering, Southern Illinois University, 6Advanced Science Research Center at the Graduate Center, City University of New York, 7Cary Institute of Ecosystem Studies, 8Rubenstein School of Environment and Natural Resources, University of Vermont, 9Department of Forestry and Natural Resources, University of Kentucky, 10Northern Research Station, U.S. Forest Service, Burlington, VT

Ice storms are important weather events that are challenging to study because of difficulties in predicting their occurrence. Here, we describe a novel method for simulating ice storms that involves spraying water over a forest canopy during sub-freezing conditions.

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JoVE Journal

Modifying Levels of Maternal Dietary Folic Acid or Choline to Study the Impact of Deficiencies on Offspring Health Outcomes
Mitra Esfandiarei 1,2,3, Teodoro Bottiglieri 4, Nafisa M. Jadavji 5,6,7
1Department of Biomedical Sciences, Midwestern University, 2Department of Basic Medical Sciences, College of Medicine Phoenix, University of Arizona, 3Department of Anesthesiology, Pharmacology, & Therapeutics, Faculty of Medicine, University of British Columbia, 4Center of Metabolomics, Institute of Metabolic Disease, Baylor Scott & White Research Institute, 5Department of Physiology, Southern Illinois University, 6Department of Child Health, College of Medicine Phoenix, University of Arizona, 7Department of Neuroscience, Carleton University

This protocol describes the method for reducing dietary intake of folic acid or choline in female mice prior to pregnancy with the objective of investigating the impact of maternal diet on offspring health outcomes.

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