University Hospital Zürich

4 ARTICLES PUBLISHED IN JoVE

Medicine

Roux-en-Y Gastric Bypass Operation in Rats

Marco Bueter^1,2, Kathrin Abegg^2,3, Florian Seyfried⁴, Thomas A. Lutz^2,3, Carel W. le Roux⁴

¹Department of Surgery, University Hospital Zürich, ²Zürich Centre for Integrative Human Physiology, University of Zürich, ³Institute of Veterinary Physiology, Vetsuisse Faculty, University of Zürich, ⁴Imperial Weight Centre, Department of Investigative Medicine, Imperial College London

Numerous studies using gastric bypass rat models have been recently conducted to uncover the underlying physiological mechanisms of Roux-en-Y gastric bypass operations. This article aims to demonstrate and discuss the technical and experimental details of our published gastric bypass rat model to understand advantages and limitations of this experimental tool.

Bioengineering

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Thilo L. Schenck¹, Myra N. Chávez¹, Alexandru P. Condurache², Ursula Hopfner¹, Farid Rezaeian³, Hans-Günther Machens¹, José T. Egaña^1,4

¹Department of Plastic Surgery and Hand Surgery, University Hospital rechts der Isar, Technische Universität München, ²Institute for Signal Processing, University of Lübeck, ³Department of Plastic Surgery and Hand Surgery, University Hospital Zürich, ⁴FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile

Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.

Medicine

Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia

Yves Harder¹, Daniel Schmauss¹, Reto Wettstein², José T. Egaña¹, Fabian Weiss¹, Andrea Weinzierl¹, Anna Schuldt¹, Hans-Günther Machens¹, Michael D. Menger³, Farid Rezaeian⁴

¹Department of Plastic Surgery and Hand Surgery, Klinikum rechts der Isar, Technische Universität München, ²Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel, ³Institute for Clinical and Experimental Surgery, University of Saarland, ⁴Division of Plastic and Hand Surgery, University Hospital Zurich

The window of the murine dorsal skinfold chamber presented visualizes a zone of acute persistent ischemia of a musculocutaneous flap. Intravital epi-fluorescence microscopy permits for direct and repetitive assessment of the microvasculature and quantification of hemodynamics. Morphologic and hemodynamic results can further be correlated with histological and molecular analyses.

Medicine

Surgical Approach, Challenges, and Resolutions for Uterus Transplantation in Rats

Keyue Sun¹, Daniela Bochicchio¹, Pierre-Alain Clavien¹, Philipp Dutkowski¹, Bostjan Humar¹

¹Swiss HPB Laboratory, Department of Visceral & Transplant Surgery, University Hospital Zürich

The present protocol describes all the essential steps for successful uterine transplantation (UTx) in rats. The rat model has proven suitable to promote the clinical implementation of UTx; however, rat UTx is a highly complex procedure requiring careful instructions.