Boston University Medical Center

2 ARTICLES PUBLISHED IN JoVE

Biology

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

Alenka Lovy¹, Anthony J.A. Molina², Fernanda M. Cerqueira³, Kyle Trudeau³, Orian S. Shirihai³

¹Department of Neuroscience, Center for Neuroscience Research, Tufts School of Medicine, ²Department of Internal Medicine, Geriatrics & Gerontology, Wake Forest Baptist Medical Center, ³Department of Medicine, Boston University Medical Center

Mitochondrial fusion was measured by tracking the equilibration of photoconverted matrix-targeted GFP across the mitochondrial network over time. Thus far, only one cell could be subjected to an hour long kinetic analysis at a time. We present a method that simultaneously measures multiple cells, thereby speeding up the data collection process.

Behavior

Chronic Social Defeat Stress in Early Adolescent Male Mice

Miranda R. Mayeaux¹, Emily L. Newman², Kerry J. Ressler², Erin E. Hisey^1,2,3

¹VA Boston, ²McLean Hospital, ³Boston University Medical Center

This protocol details how to perform 10 day chronic social defeat stress in early adolescent male mice to produce long-lasting social avoidance in adulthood in more than 60% of mice.