The Scripps Research Institute
2 ARTICLES PUBLISHED IN JoVE
Biology
Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
Kim Titus *1, Vitaly Klimovich *2, Mark Rothenberg 2, Pilar Pardo *2, Allison Tanner *3, Greg Martin 3
1Business Development, Corning Life Science, 2Applications, Corning Life Science, 3Product Development, Corning Life Science
An Introduction into the technology, protocol and handling of the Corning HYPERStack Vessels and accessories used for high yield adherent cell culture. The protocol will show how to use the closed system vessels for increasing cell harvesting over current stacked plate products.
Biology
Generation of Mice Derived from Induced Pluripotent Stem Cells
Michael J. Boland 1, Jennifer L. Hazen 1, Kristopher L. Nazor 1, Alberto R. Rodriguez 2, Greg Martin 2, Sergey Kupriyanov 2, Kristin K. Baldwin 1
1Dorris Neuroscience Center & Department of Cell Biology, The Scripps Research Institute , 2Mouse Genetics Core Facility, The Scripps Research Institute
Generating induced pluripotent stem cell (iPSC) lines produces lines of differing developmental potential even when they pass standard tests for pluripotency. Here we describe a protocol to produce mice derived entirely from iPSCs, which defines the iPSC lines as possessing full pluripotency1.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved