Albert Einstein College of Medicine, Yeshiva University

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Revealing Neural Circuit Topography in Multi-Color

Stacey L. Reeber¹, Samrawit A. Gebre¹, Nika Filatova¹, Roy V. Sillitoe¹

¹Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University

We provide a practical guide for delivering tracers in vivo and use the spinocerebellar pathway as a model system to demonstrate essential steps for successful neuronal circuit analysis in mice. We describe in detail our versatile tracing protocol that exploits wheat germ agglutinin (WGA) conjugated to Alexa fluorophores.

Neuroscience

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

Joshua J. White¹, Stacey L. Reeber¹, Richard Hawkes², Roy V. Sillitoe¹

¹Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University , ²Department of Cell Biology and Anatomy and the Hotchkiss Brain Institute, Faculty of Medicine, University of Calgary

Neural circuits are topographically organized into functional compartments with specific molecular profiles. Here, we provide the practical and technical steps for revealing global brain topography using a versatile wholemount immunohistochemical staining approach. We demonstrate the utility of the method using the well-understood cytoarchitecture and circuitry of cerebellum.