JoVE Logo

Sign In

College of William and Mary

4 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
Molly J. McDonough *1, Chelsea E. Allen *1, Ng-Kwet-Leok A. Ng-Sui-Hing 1, Brian A. Rabe 1, Brittany B. Lewis 1, Margaret S. Saha 1
1Department of Biology, College of William and Mary

Xenopus laevis provides an ideal model system for studying cell fate specification and physiological function of individual retinal cells in primary cell culture. Here we present a technique for dissecting retinal tissues and generating primary cell cultures that are imaged for calcium activity and analyzed by in situ hybridization.

image

Chemistry

Conducting Reactions Below Room Temperature
Dana Lashley 1
1College of William and Mary

Conducting Reactions Below Room Temperature

image

Biology

Dissection and Downstream Analysis of Zebra Finch Embryos at Early Stages of Development
Jessica R. Murray 1, Monika E. Stanciauskas 1, Tejas S. Aralere 1, Margaret S. Saha 1
1Department of Biology, College of William and Mary

The zebra finch (Taeniopygiaguttata) is a valuable model organism; however, early stages of zebra finch development have not been extensively studied. The protocol describes how to dissect early embryos for developmental and molecular applications.

image

Developmental Biology

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
Eileen F. Ablondi 1, Sudip Paudel 2, Morgan Sehdev 3, John P. Marken 4, Andrew D. Halleran 4, Atiqur Rahman 5, Peter Kemper 5, Margaret S. Saha 2
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University, 2Department of Biology, College of William and Mary, 3Harvard Medical School, Harvard University, 4Department of Bioengineering, California Institute of Technology, 5Department of Computer Science, College of William and Mary

We present a two-part protocol that combines fluorescent calcium imaging with in situ hybridization, allowing the experimenter to correlate patterns of calcium activity with gene expression profiles on a single-cell level.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved