Upstate Medical University

2 ARTICLES PUBLISHED IN JoVE

Biology

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Guangliang Wang¹, H. Joseph Yost², Jeffrey D. Amack¹

¹Department of Cell and Developmental Biology, State University of New York, Upstate Medical University, ²Department of Neurobiology and Anatomy, Eccles Institute of Human Genetics, University of Utah

Cilia-generated fluid flow in Kupffer’s Vesicle (KV) controls left-right patterning of the zebrafish embryo. Here, we describe a technique to modulate gene function specifically in KV cells. In addition, we show how to deliver fluorescent beads into KV to visualize fluid flow.

Medicine

Multi-system Monitoring for Identification of Seizures, Arrhythmias and Apnea in Conscious Restrained Rabbits

Cameron Bosinski¹, Kyle Wagner¹, Xiangping Zhou², Lynn Liu³, David S. Auerbach¹

¹Department of Pharmacology, Upstate Medical University, ²Department of Neurology, Upstate Medical University, ³Strong Epilepsy Center, Departments of Neurology, Anesthesiology / Perioperative Medicine, & Pediatrics, University of Rochester Medical Center

Using simultaneous video-EEG-ECG-oximetry-capnography, we developed a methodology to evaluate the susceptibility of rabbit models to develop provoked arrhythmias and seizures. This novel recording system establishes a platform to test the efficacy and safety of therapeutics and can capture the complex cascade of multi-system events that culminate in sudden death.