University of Western Sydney

7 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Andrew J. McElrone^1,2, Brendan Choat³, Dilworth Y. Parkinson⁴, Alastair A. MacDowell⁴, Craig R. Brodersen⁵

¹U.S. Department of Agriculture, ²Department of Viticulture and Enology, University of California - Davis, ³Hawkesbury Institute for the Environment, University of Western Sydney, ⁴Advanced Light Source, Lawrence Berkeley National Lab, ⁵Citrus Research & Education Center, University of Florida

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique that can be used to study the structure and function of plant vasculature in 3D. We demonstrate how HRCT facilitates exploration of xylem networks across a wide range of plant tissues and species.

Neuroscience

Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

Jin Y. Huang¹, Klaus M. Stiefel², Dario A. Protti³

¹Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney , ²The MARCS Institute, University of Western Sydney, ³Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney

This video article illustrates the set-up, the procedures to patch cell bodies and how to implement dynamic clamp recordings from ganglion cells in whole-mount mouse retinae. This technique allows the investigation of the precise contribution of excitatory and inhibitory synaptic inputs, and their relative magnitude and timing to neuronal spiking.

Chemistry

Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents

Michael G. Apps¹, Ben W. Johnson², Oliver B. Sutcliffe³, Sarah D. Brown⁴, Nial J. Wheate¹

¹Faculty of Pharmacy, The University of Sydney, ²School of Science and Health, University of Western Sydney, ³Division of Chemistry and Environmental Science, School of Science and the Environment, Manchester Metropolitan University, ⁴Nature Publishing Group

This protocol describes the use of amide coupling reactions of isonicotinic acid and diaminoalkanes to form bridging ligands suitable for use in the synthesis of multinuclear platinum complexes, which combine aspects of the anticancer drugs BBR3464 and picoplatin.

Chemistry

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Sercan Pravadali-Cekic¹, Danijela Kocic¹, Stanley Hua¹, Andrew Jones¹, Gary R. Dennis¹, R. Andrew Shalliker¹

¹School of Science and Health, University of Western Sydney

Here, we present a protocol for the operation and tuning of parallel segmented flow chromatography columns to enable multiplexed detection.

JoVE Core

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Andrew Jones¹, Sercan Pravadali-Cekic¹, Stanley Hua¹, Danijela Kocic¹, Michelle Camenzuli², Gary Dennis¹, Andrew Shalliker¹

¹School of Science and Health, University of Western Sydney, ²Van′t Hoff Institute for Molecular Sciences, University of Amsterdam

A protocol for the use of reaction flow high performance liquid chromatography columns for methods employing post column derivatization (PCD) is presented.

Chemistry

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Sercan Pravadali-Cekic¹, Danijela Kocic¹, Stanley Hua¹, Andrew Jones¹, Gary Dennis¹, Andrew Shalliker¹

¹School of Science and Health, University of Western Sydney

Here, we present a protocol for the operation and optimization of Active Flow Technology (AFT) column in Curtain flow (CF) mode for enhanced separation performance.

Chemistry

Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis

Sercan Pravadali-Cekic¹, Jake P. Violi¹, Simon M. Mitrovic², Kenneth J. Rodgers², David Bishop¹

¹Hyphenated Mass Spectrometry Laboratory (HyMaS), University of Technology Sydney, ²School of Life Sciences, University of Technology Sydney

The present protocol describes the extraction of non-protein amino acids from biological matrices via trichloroacetic acid (TCA) protein precipitation and acid hydrolysis before analysis using liquid chromatography-tandem mass spectrometry.