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University of Western Sydney

7 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature
Andrew J. McElrone 1,2, Brendan Choat 3, Dilworth Y. Parkinson 4, Alastair A. MacDowell 4, Craig R. Brodersen 5
1U.S. Department of Agriculture, 2Department of Viticulture and Enology, University of California - Davis, 3Hawkesbury Institute for the Environment, University of Western Sydney, 4Advanced Light Source, Lawrence Berkeley National Lab, 5Citrus Research & Education Center, University of Florida

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique that can be used to study the structure and function of plant vasculature in 3D. We demonstrate how HRCT facilitates exploration of xylem networks across a wide range of plant tissues and species.

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Neuroscience

Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
Jin Y. Huang 1, Klaus M. Stiefel 2, Dario A. Protti 3
1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney , 2The MARCS Institute, University of Western Sydney, 3Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney

This video article illustrates the set-up, the procedures to patch cell bodies and how to implement dynamic clamp recordings from ganglion cells in whole-mount mouse retinae. This technique allows the investigation of the precise contribution of excitatory and inhibitory synaptic inputs, and their relative magnitude and timing to neuronal spiking.

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Chemistry

Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents
Michael G. Apps 1, Ben W. Johnson 2, Oliver B. Sutcliffe 3, Sarah D. Brown 4, Nial J. Wheate 1
1Faculty of Pharmacy, The University of Sydney, 2School of Science and Health, University of Western Sydney, 3Division of Chemistry and Environmental Science, School of Science and the Environment, Manchester Metropolitan University, 4Nature Publishing Group

This protocol describes the use of amide coupling reactions of isonicotinic acid and diaminoalkanes to form bridging ligands suitable for use in the synthesis of multinuclear platinum complexes, which combine aspects of the anticancer drugs BBR3464 and picoplatin.

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Chemistry

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
Sercan Pravadali-Cekic 1, Danijela Kocic 1, Stanley Hua 1, Andrew Jones 1, Gary R. Dennis 1, R. Andrew Shalliker 1
1School of Science and Health, University of Western Sydney

Here, we present a protocol for the operation and tuning of parallel segmented flow chromatography columns to enable multiplexed detection.

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Chemistry

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Andrew Jones 1, Sercan Pravadali-Cekic 1, Stanley Hua 1, Danijela Kocic 1, Michelle Camenzuli 2, Gary Dennis 1, Andrew Shalliker 1
1School of Science and Health, University of Western Sydney, 2Van′t Hoff Institute for Molecular Sciences, University of Amsterdam

A protocol for the use of reaction flow high performance liquid chromatography columns for methods employing post column derivatization (PCD) is presented.

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Chemistry

Curtain Flow Column: Optimization of Efficiency and Sensitivity
Sercan Pravadali-Cekic 1, Danijela Kocic 1, Stanley Hua 1, Andrew Jones 1, Gary Dennis 1, Andrew Shalliker 1
1School of Science and Health, University of Western Sydney

Here, we present a protocol for the operation and optimization of Active Flow Technology (AFT) column in Curtain flow (CF) mode for enhanced separation performance.

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Chemistry

Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis
Sercan Pravadali-Cekic 1, Jake P. Violi 1, Simon M. Mitrovic 2, Kenneth J. Rodgers 2, David Bishop 1
1Hyphenated Mass Spectrometry Laboratory (HyMaS), University of Technology Sydney, 2School of Life Sciences, University of Technology Sydney

The present protocol describes the extraction of non-protein amino acids from biological matrices via trichloroacetic acid (TCA) protein precipitation and acid hydrolysis before analysis using liquid chromatography-tandem mass spectrometry.

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