Lawrence Berkeley National Lab

5 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Andrew J. McElrone^1,2, Brendan Choat³, Dilworth Y. Parkinson⁴, Alastair A. MacDowell⁴, Craig R. Brodersen⁵

¹U.S. Department of Agriculture, ²Department of Viticulture and Enology, University of California - Davis, ³Hawkesbury Institute for the Environment, University of Western Sydney, ⁴Advanced Light Source, Lawrence Berkeley National Lab, ⁵Citrus Research & Education Center, University of Florida

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique that can be used to study the structure and function of plant vasculature in 3D. We demonstrate how HRCT facilitates exploration of xylem networks across a wide range of plant tissues and species.

Engineering

Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography

Katherine J. Harry^1,2, Dilworth Y. Parkinson³, Nitash P. Balsara^2,4,5

¹Department of Materials Science and Engineering, University of California Berkeley, ²Materials Science Division, Lawrence Berkeley National Laboratory, ³Advanced Light Source Division, Lawrence Berkeley National Laboratory, ⁴Department of Chemical and Biomolecular Engineering, University of California Berkeley, ⁵Environmental Energy Technology Division, Lawrence Berkeley National Laboratory

Synchrotron-based hard X-ray microtomography is used to image the electrochemical growth of dendrites from a lithium metal electrode through a solid polymer electrolyte membrane.

Engineering

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Holly D. Carlton¹, John W. Elmer¹, Yan Li², Mario Pacheco², Deepak Goyal², Dilworth Y. Parkinson³, Alastair A. MacDowell³

¹Materials Engineering Division, Lawrence Livermore National Laboratory, ²Assembly Test and Technology Development Failure Analysis Labs, Intel Corporation, ³Advanced Light Source, Lawrence Berkeley National Laboratory

For this study synchrotron radiation micro-tomography, a non-destructive three-dimensional imaging technique, is employed to investigate an entire microelectronic package with a cross-sectional area of 16 x 16 mm. Due to the synchrotron's high flux and brightness the sample was imaged in just 3 min with an 8.7 µm spatial resolution.

Bioengineering

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Camila Hochman-Mendez¹, Tatiana Coelho-Sampaio², Ariel J. Kent³, Jamie L. Inman³, Mina J. Bissell³, Claire Robertson⁴

¹Regenerative Medicine Research, Texas Heart Institute, ²Institute of Biomedical Sciences, Federal University of Rio de Janeiro, ³Biological Systems and Engineering, Lawrence Berkeley National Lab, ⁴Materials Engineering Division, Lawrence Livermore National Lab

We describe three methods to generate Ln1 polymers with fractal properties that signal to cells differently compared to unpolymerized Ln1.

JoVE Core

Determining the Mechanical Strength of Ultra-Fine-Grained Metals

Jianing Xu^*1,2, Yanju Wang^*2, Jinyuan Yan³, Bin Chen^1,2

¹School of Science, Harbin Institute of Technology, ²Center for High Pressure Science and Technology Advanced Research, ³Advanced Light Source, Lawrence Berkeley National Lab

The protocol presented here describes the high-pressure radial diamond-anvil-cell experiments and analyzing the related data, which are essential for obtaining the mechanical strength of the nanomaterials with a significant breakthrough to the traditional approach.