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Louisiana State University Health Science Center

3 ARTICLES PUBLISHED IN JoVE

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Biology

Chromosome Preparation From Cultured Cells
Bradley Howe 1, Ayesha Umrigar 1, Fern Tsien 1
1Department of Genetics, Louisiana State University Health Science Center

Chromosomes can be isolated from live cells such as lymphocytes or skin fibroblasts, and from organisms including humans or mice. These chromosome preparations can be further utilized for routine G-banding and molecular cytogenetic procedures such as fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and spectral karyotyping (SKY).

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Biology

The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
Luke A. White 1, Emily V. Stevenson 1, J. Winny Yun 1, Randa Eshaq 1, Norman R. Harris 1, David K. Mills 2, Alireza Minagar 3, Pierre-Olivier Couraud 4, J. Steven Alexander 1
1Molecular and Cellular Physiology, Louisiana State University Health Sciences Center in Shreveport, 2Biological Sciences, Louisiana Tech University, 3Neurology, Louisiana State University Health Sciences Center in Shreveport, 4Institut Cochin, Inserm U1016, Cnrs Umr8104, Université Paris Descartes

Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.

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Behavior

Utilizing the Modified T-Maze to Assess Functional Memory Outcomes After Cardiac Arrest
Celeste Y. C. Wu 1,2, Francesca M. Lerner 4, Alexandre Couto e Silva 3, Harlee E. Possoit 1,2, Tsung-Han Hsieh 1,2, Jake T. Neumann 5, Alireza Minagar 1, Hung Wen Lin 1,2,3, Reggie H. C. Lee 1,2
1Department of Neurology, Louisiana State University Health Science Center, 2Center for Brain Health, Louisiana State University Health Science Center, 3Department of Cellular Biology and Anatomy, Louisiana State University Health Science Center, 4Department of Neurology, Cerebral Vascular Disease Research Laboratories, University of Miami Miller School of Medicine, 5Department of Biomedical Sciences, West Virginia School of Osteopathic Medicine

This protocol describes the use of a modified T-maze to evaluate functional learning/memory in asphyxia cardiac arrest-induced cerebral ischemia.

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