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University of St Andrews

5 ARTICLES PUBLISHED IN JoVE

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Biology

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
Alexander D. Leeper 1, Joanne Farrell 2, J. Michael Dixon 1, Sarah E. Wedden 2, David J. Harrison 1, Elad Katz 1
1Breakthrough Breast Cancer Research Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, 2MRC Technology

We have developed a collagen-based in vitro assay which promotes proliferation and invasion from samples of all breast cancer subtypes. Optical Projection Tomography, a three dimensional microscopy technique was utilised to visualise and quantify tumour expansion. This assay may be used to quantify drug response of individual tumour samples.

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Medicine

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence
Dana Faratian 1, Jason Christiansen 2, Mark Gustavson 2, Christine Jones 2, Christopher Scott 2, InHwa Um 1, David J. Harrison 1
1Division of Pathology, University of Edinburgh, 2HistoRx Inc.

Here we describe a method to quantify molecular heterogeneity in histological sections of tumor material using quantitative immunofluorescence, image analysis, and a statistical measure of heterogeneity. The method is intended for use in clinical biomarker development and analysis.

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Engineering

Fabrication And Characterization Of Photonic Crystal Slow Light Waveguides And Cavities
Christopher Paul Reardon 1, Isabella H. Rey 1, Karl Welna 1, Liam O'Faolain 1, Thomas F. Krauss 1
1School of Physics & Astronomy, University of St Andrews

Use of photonic crystal slow light waveguides and cavities has been widely adopted by the photonics community in many differing applications. Therefore fabrication and characterization of these devices are of great interest. This paper outlines our fabrication technique and two optical characterization methods, namely: interferometric (waveguides) and resonant scattering (cavities).

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Medicine

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
Fiach C. O'Mahony 1, Jyoti Nanda 1, Alexander Laird 1, Peter Mullen 2, Helen Caldwell 3, Ian M. Overton 4, Lel Eory 4, Marie O'Donnell 1,5, Dana Faratian 6, Thomas Powles 7, David J. Harrison 1,2, Grant D. Stewart 1
1Edinburgh Urological Cancer Group, University of Edinburgh, 2School of Medicine, University of St Andrews, 3Division of Pathology, University of Edinburgh, 4MRC Human Genetics Unit, MRC IGMM, University of Edinburgh, 5Department of Pathology, Western General Hospital, 6Breakthrough Breast Cancer Research Unit, University of Edinburgh, 7St Bartholomew's Cancer Institute, Experimental Cancer Medicine Centre, Queen Mary University of London

RPPA enables the protein expression of hundreds of samples, printed on nitrocellulose slides to be interrogated simultaneously, using fluorescently labelled antibodies. This technique has been applied to study the effect of drug treatment heterogeneity within clear cell renal carcinoma.

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Immunology and Infection

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
Wilber Sabiiti 1, Bariki Mtafya 1,2, Daniela Alferes De Lima 1, Evelin Dombay 1, Vincent O. Baron 1, Khalide Azam 3, Katarina Oravcova 4, Derek J. Sloan 1, Stephen H. Gillespie 1
1Division of Infection and Global Health, School of Medicine, University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow

We describe a tuberculosis molecular bacterial load assay test performed after heat inactivation of sputum. Heat inactivation renders sputum samples noninfectious and obviates the need for containment level 3 laboratories for tuberculosis molecular tests.

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