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University of Heidelberg

5 ARTICLES PUBLISHED IN JoVE

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Biology

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
Niels Voigt *1,2, Xiao-Bo Zhou *2, Dobromir Dobrev 1,2
1Institute of Pharmacology, University of Duisburg-Essen , 2Division of Experimental Cardiology, University of Heidelberg

We describe the isolation of human atrial myocytes which can be used for intracellular Ca2+ measurements in combination with electrophysiological patch-clamp studies.

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Immunology and Infection

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
Christian Erbel 1, Gregor Rupp 1, Christian M. Helmes 1, Mirjam Tyka 1, Fabian Linden 1, Andreas O. Doesch 1, Hugo A. Katus 1, Christian A. Gleissner 1
1Department of Cardiology, University of Heidelberg

Monocyte-derived macrophages are important cells of the innate immune system. Here, we describe an easy to use in vitro model to generate these cells. Using gradient centrifugation, negative bead isolation and specific cell culture conditions, monocyte-derived macrophages can be generated for phenotypic and functional studies.

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Neuroscience

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
Roberta Haddad-Tóvolli *1, Nora-Emöke Szabó *2, Xunlei Zhou 1, Gonzalo Alvarez-Bolado 1
1Institute of Anatomy and Cell Biology, University of Heidelberg , 2Institut de recherches cliniques de Montreal

Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.

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Medicine

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology
Christian Erbel *1, Deniz Okuyucu *1, Mohammadreza Akhavanpoor 1, Li Zhao 1, Susanne Wangler 1, Maani Hakimi 2, Andreas Doesch 1, Thomas J. Dengler 3, Hugo A. Katus 1, Christian A. Gleissner 1
1Department of Cardiology, University of Heidelberg, 2Department of Vascular Surgery, University of Heidelberg, 3Department of Cardiology, SLK Hospital am Plattenwald

Atherosclerosis is a chronic inflammatory process. This manuscript illustrates an easy to use ex vivo model to investigate fresh carotid or coronary artery plaques. The ex vivo model allows for the investigation of potential substances on the inflammatory milieu in human atherosclerotic lesions and results can be analyzed by various methods.

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Bioengineering

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Fitzwilliam Seibertz 1,2, Martyn Reynolds 3, Niels Voigt 1,2,4
1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, Goettingen, Germany, 2DZHK (German Center for Cardiovascular Research), Partner Site Goettingen, Germany, 3Cairn Research Ltd, Faversham, United Kingdom, 4Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Goettingen, Germany

Here we describe optical acquisition and characterization of action potentials from induced pluripotent stem cell derived cardiomyocytes using a high-speed modular photometry system.

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