Hôtel-Dieu de Québec

2 ARTICLES PUBLISHED IN JoVE

Biology

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins

Ranjan Maity^*1, Joris Pauty^*1, Jana Krietsch^*1, Rémi Buisson¹, Marie-Michelle Genois¹, Jean-Yves Masson¹

¹Genome Stability Laboratory, Oncology Axis, Hôtel-Dieu de Québec

In the present protocol, we demonstrate a highly efficient and cost-effective small-scale protein purification method, which allows purification of recombinant proteins by uniquely combining a cleavable GST-tag and a small His-tag.

Biology

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Julia O'Sullivan^*1,2, Sofiane Y. Mersaoui^*1,2, Guy Poirier^2,3, Jean-Yves Masson^1,2

¹Oncology Division, Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, ²Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University Cancer Research Center, ³Oncology Division, CHU de Québec Research Center, CHUL Pavilion

In the present protocol, we demonstrate how to visualize DNA double-strand end resection during S/G2 phase of the cell cycle using an immunofluorescence-based method.