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Washington University in St. Louis School of Medicine

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK
Jessica Morris 1, Patrick M. Stuart 1, Megan Rogge 1, Chloe Potter 1, Nipun Gupta 1, Xiao-Tang Yin 1
1Department of Ophthalmology, Saint Louis University

Most studies of herpetic corneal disease use a primary infection model. However, primary infection with HSV-1 does not typically lead to human disease. Here we describe a recurrent model of herpetic corneal disease, which more closely mimics human disease.

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Neuroscience

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
Sarah L. DeVos 1, Timothy M. Miller 1
1Department of Neurology, Washington University in St. Louis School of Medicine

We describe a method to target drugs to the central nervous system by either implanting a catheter or performing a bolus injection into the right lateral ventricle in mice. We focus specifically on the delivery of antisense oligonucleotides. This technique is readily adaptable to other drugs and to rats.

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Biology

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
David Razafsky 1, Chloe Potter 1, Didier Hodzic 1
1Department of Ophthalmology and Visual Sciences, Washington University in St. Louis School of Medicine

Nuclear envelope proteins play a central role in many basic biological processes and have been implicated in a variety of human diseases. This protocol describes a new Cre/Lox-based mouse model that allows for the spatiotemporal control of LINC complexes disruption.

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Biology

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Tyler M. Bauman 1,2, Emily A. Ricke 2, Sally A. Drew 3, Wei Huang 3,4, William A. Ricke 2,4
1Division of Urologic Surgery, Washington University in St. Louis School of Medicine, 2Department of Urology, University of Wisconsin School of Medicine and Public Health, 3Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, 4O’Brien Urology Research Center, University of Wisconsin School of Medicine and Public Health

Immunohistochemistry is a powerful lab technique for evaluating protein localization and expression within tissues. Current semi-automated methods for quantitation introduce subjectivity and often create irreproducible results. Herein, we describe methods for multiplexed immunohistochemistry and objective quantitation of protein expression and co-localization using multispectral imaging.

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Neuroscience

Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
Brandon A. Miller 1,2, Shelei Pan 3, Peter H. Yang 3, Catherine Wang 1, Amanda L. Trout 1, Dakota DeFreitas 3, Sruthi Ramagiri 3, Scott D. Olson 2, Jennifer M. Strahle 3,4,5
1Department of Neurosurgery, University of Kentucky, 2Department of Pediatric Surgery, University of Texas, 3Department of Neurological Surgery, Washington University in St. Louis School of Medicine, 4Department of Orthopedic Surgery, Washington University in St. Louis School of Medicine, 5Department of Pediatrics, Washington University in St. Louis School of Medicine

We present a model of neonatal intraventricular hemorrhage using rat pups that mimics the pathology seen in humans.

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