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Norwegian University of Life Sciences

8 ARTICLES PUBLISHED IN JoVE

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Neuroscience

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)
Ying Wang 1, Nicholas Baker 1, Gro V. Amdam 1,2
1School of Life Sciences, Arizona State University , 2Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences

In this protocol, we describe two strategies that simultaneously suppress two genes (double gene knockdown) in honey bees. Then we present how to use the proboscis extension response (PER) assay to study the effect of double gene knockdown on honey bee gustatory perception.

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Biology

Obtaining Specimens with Slowed, Accelerated and Reversed Aging in the Honey Bee Model
Daniel Münch 1, Nicholas Baker 2, Erik M.K. Rasmussen 1, Ashish K. Shah 1, Claus D. Kreibich 1, Lars E. Heidem 1, Gro V. Amdam 1,2
1Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 2School of Life Sciences, Arizona State University

In honey bee workers, aging depends on social behaviors rather than on chronological age. Here we show how worker-types with very different aging patterns can be obtained and analyzed for cellular senescence.

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Behavior

Tickling, a Technique for Inducing Positive Affect When Handling Rats
Sylvie Cloutier 1,2, Megan R. LaFollette 3, Brianna N. Gaskill 3, Jaak Panksepp 1, Ruth C. Newberry 4
1Center for the Study of Animal Well-being, Department of Integrative Physiology and Neuroscience, Washington State University, 2Canadian Council on Animal Care, 3Department of Animal Sciences, Center for Animal Welfare Science, College of Agriculture, Purdue University, 4Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences

This article demonstrates the standardized application of playful handling, a tickling technique designed to mimic rat rough-and-tumble play. This technique is effective at reducing fearful reactions to humans and generating positive affect when rats are handled for common husbandry activities and medical and research procedures such as injection.

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Neuroscience

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
Romain Fontaine *1, Kjetil Hodne *1, Finn-Arne Weltzien 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences

The article describes an optimized protocol for making viable brain-pituitary tissue slices, using the teleost fish medaka (Oryzias latipes), followed by electrophysiological recordings of pituitary cells using the patch-clamp technique with the perforated patch configuration.

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Neuroscience

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
Eirill Ager-Wick 1, Kjetil Hodne 1, Romain Fontaine 1, Kristine von Krogh 1, Trude M. Haug 2, Finn-Arne Weltzien 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 2Department of Oral Biology, Faculty of Dentistry, University of Oslo

Here we describe a protocol to prepare and maintain primary pituitary cell cultures from medaka (Oryzias latipes). The optimized conditions in this protocol take important parameters such as temperature, osmolality, and pH into consideration by mimicking the physiological conditions of the fish, thereby enabling physiologically more meaningful results.

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Bioengineering

Labeling of Blood Vessels in the Teleost Brain and Pituitary Using Cardiac Perfusion with a DiI-fixative
Romain Fontaine 1, Finn-Arne Weltzien 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences

The article describes a quick protocol for labeling blood vessels in a teleost fish by cardiac perfusion of DiI diluted in fixative, using medaka (Oryzias latipes) as a model and focusing on brain and pituitary tissue.

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Biology

Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)
Muhammad Rahmad Royan 1, Shinji Kanda 2, Daichi Kayo 3, Weiyi Song 4, Wei Ge 4, Finn-Arne Weltzien 1, Romain Fontaine 1
1Physiology Unit, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 2Laboratory of Physiology, Atmosphere and Ocean Research Institute, The University of Tokyo, 3Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 4Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau

The article describes a quick protocol to gonadectomize and sample blood from the small teleost fish, using Japanese medaka (Oryzias latipes) as a model, to investigate the role of sex steroids in animal physiology.

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Biology

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)
Lauren Closs 1, Amin Sayyari 2, Romain Fontaine 1
1Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 2Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences

This article describes two quick and efficient methods for collecting sperm from the small model fish medaka (Oryzias latipes), as well as a protocol for reliably assessing sperm quality using computer-assisted sperm analysis (CASA).

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