R&D Systems, Inc.

2 ARTICLES PUBLISHED IN JoVE

Profiling Changes in Receptor Tyrosine Kinase Phosphorylation using Antibody Arrays - ADVERTISEMENT

Greta J. Wegner¹, David J. Finkel¹, Amy E. James¹, Richard A. Krzyzek¹

¹Array Group, Assay Department, R&D Systems, Inc.

Proteome Profiler antibody arrays are a convenient and cost efficient way to screen for changes in receptor tyrosine kinase (RTK) phosphorylation without performing numerous immunoprecipitation (IP) Westerns. The ARY001 Human RTK array allows for the qualitative measurement of multiple RTKs in a single sample using chemiluminescence detection.

Biology

Induction and Analysis of Epithelial to Mesenchymal Transition

Yixin Tang¹, Greg Herr², Wade Johnson², Ernesto Resnik¹, Joy Aho²

¹Antibody Development Department, R&D Systems, Inc., ²Stem Cells and Developmental Biology Department, R&D Systems, Inc.

A straightforward method is described for the induction of epithelial to mesenchymal transition (EMT) in a variety of cell types. Methods for the analysis of cells in EMT states by immunocytochemistry are included.