Hôpitaux Universitaires de Strasbourg

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo

Nihal E. Vrana¹, Agnes Dupret-Bories^1,2, Christophe Chaubaroux¹, Elisabeth Rieger^1,2, Christian Debry^1,2, Dominique Vautier^1,3, Marie-Helene Metz-Boutigue^1,3, Philippe Lavalle^1,3

¹Biomatériaux et Bioingénieriee, INSERM, ²Service Oto-Rhino-Laryngologie, Hôpitaux Universitaires de Strasbourg, ³Faculté de Chirurgie Dentaire, Université de Strasbourg

In this video, we will demonstrate modification techniques for porous metallic implants to improve their functionality and to control cell migration. Techniques include development of pore gradients to control cell movement in 3D and production of basement membrane mimics to control cell movement in 2-D. Also, a HPLC-based method for monitoring implant integration in-vivo via analysis of blood proteins is described.

Medicine

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice

Ipek Yalcin¹, Salim Megat^1,2, Florent Barthas^1,2, Elisabeth Waltisperger¹, Mélanie Kremer^1,2, Eric Salvat^1,2,3, Michel Barrot¹

¹Institut des Neurosciences Cellulaires et Intégratives UPR3212, Centre National de la Recherche Scientifique, ²Université de Strasbourg, ³Hôpitaux Universitaires de Strasbourg

Neuropathic pain is a consequence of a lesion or disease affecting the somatosensory system. The “cuff model” of neuropathic pain in mice consists of the implantation of a polyethylene cuff around the main branch of the sciatic nerve. Mechanical allodynia is tested using von Frey filaments.

Biology

FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.

Hanna Manko¹, Vincent Normant^2,3, Quentin Perraud^2,3, Tania Steffan¹, Véronique Gasser^2,3, Emmanuel Boutant¹, Éléonore Réal¹, Isabelle J. Schalk^2,3, Yves Mély¹, Julien Godet^1,4

¹Université de Strasbourg, Laboratoire de Bioimagerie et Pathologies, UMR CNRS 7021, ²Université de Strasbourg, UMR 7242, ESBS, ³CNRS, UMR 7242, ESBS, ⁴Groupe Méthode Recherche Clinique, Hôpitaux Universitaires de Strasbourg

We describe here a protocol to characterize protein-protein interactions between two highly-differently expressed proteins in live Pseudomonas aeruginosa using FLIM-FRET measurements. The protocol includes bacteria strain constructions, bacteria immobilization, imaging and post-imaging data analysis routines.