Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain InjuryCarlo Perego *1, Stefano Fumagalli *1, Maria-Grazia De Simoni 1
1Department of Neuroscience, IRCCS - Istituto di Ricerche Farmacologiche Mario Negri
A way to gain new insights into the complexity of the brain inflammatory response is presented. We describe immunofluorescence-based protocols followed by three-dimensional confocal analysis to investigate the pattern of co-expression of microglia/macrophage phenotype markers in a mouse model of focal ischemia.
