Hôpital X. Bichat, AP-HP, Paris

2 ARTICLES PUBLISHED IN JoVE

Medicine

A New Murine Model of Endovascular Aortic Aneurysm Repair

Martin Rouer¹, Olivier Meilhac¹, Sandrine Delbosc¹, Liliane Louedec¹, Graciela Pavon-Djavid^1,2, Jane Cross³, Josette Legagneux⁴, Maxime Bouilliant-Linet⁴, Jean-Baptiste Michel¹, Jean-Marc Alsac^1,5

¹INSERM U698 Cardiovascular Remodelling, Hôpital X. Bichat, AP-HP, Paris, ²Bio-Ingénierie des Polymères Cardiovasculaires (BPC), Institut Galilée - Université Paris 13, Paris, France, ³Service de Chirurgie Vasculaire, Hôpital Henri Mondor, AP-HP, Université Paris-Est Creteil, ⁴Ecole de chirurgie de l'assistance publique des hôpitaux de Paris, ⁵Service de Chirurgie Cardiaque et Vasculaire, Hôpital Européen Georges Pompidou, AP-HP, Université René Descartes

Histological and biochemical modifications after aneurysm endograft exclusion are still unclear. We describe a new model of endograft implantation on a murine aneurysm. Through thrombus analysis with and without persistant circulating blood stream interface, and new endovascular biomaterials evaluation, this model will help to better understand endovascular aneurysm exclusion pathobiology.

Developmental Biology

Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules

Anne-Claire Dorsemans¹, Christian Lefebvre d'Hellencourt¹, Imade Ait-Arsa², Emmanuelle Jestin^1,2, Olivier Meilhac^1,3, Nicolas Diotel¹

¹UMR 1188 Diabète athérothombose Thérapie Réunion Océan Indien (DéTROI), Saint-Denis de La Réunion, France, Université de La Réunion, INSERM, ²RIPA (Radiochimie et Imagerie du Petit Animal), Cyclotron de la Réunion Océan Indien CYROI, ³CHU de La Réunion

This work describes methods to establish acute and chronic hyperglycemia models in zebrafish. The aim is to investigate the impact of hyperglycemia on physiological processes, such as constitutive and injury-induced neurogenesis. The work also highlights the use of zebrafish to follow radiolabeled molecules (here, [¹⁸F]-FDG) using PET/CT.