CNRS UMR 5534

2 ARTICLES PUBLISHED IN JoVE

Biology

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

Marie-Christine Mariol^1,2, Ludivine Walter^1,2, Stéphanie Bellemin^1,2, Kathrin Gieseler^1,2

¹Université Claude Bernard Lyon, ²Centre de Génétique et de Physiologie Moléculaires et Cellulaires, CNRS UMR 5534

This protocol describes a rapid and low material consuming procedure for the integration of transgenic extrachromosomal arrays into the Caenorhabditis elegans genome using ultra violet (UV) irradiation. Furthermore, this protocol is particularly well suited for transgenic lines that transmit extrachromosomal arrays at a high rate.

Neuroscience

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Frédéric Catez^1,2,5, Antoine Rousseau⁴, Marc Labetoulle⁴, Patrick Lomonte^1,2,3

¹Virus and Centromere Team, Centre de Génétique et Physiologie Moléculaire et Cellulaire, CNRS UMR 5534, ²Université de Lyon 1, ³Laboratoire d'excellence, LabEX DEVweCAN, ⁴Institut de Virologie Moléculaire et Structurale, CNRS UPR 3296, ⁵Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR 5286

We established a fluorescent in situ hybridization protocol for the detection of a persistent DNA virus genome within tissue sections of animal models. This protocol enables studying infection process by codetection of the viral genome, its RNA products, and viral or cellular proteins within single cells.