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University of Illinois Urbana-Champaign

17 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Brain Imaging Investigation of the Neural Correlates of Observing Virtual Social Interactions
Keen Sung 1, Sanda Dolcos 2, Sophie Flor-Henry 3, Crystal Zhou 3, Claudia Gasior 4, Jennifer Argo 5, Florin Dolcos 2,6,7
1Department of Computing Science, University of Alberta, 2Department of Psychology, University of Illinois, 3Centre for Neuroscience, University of Alberta, 4Department of Psychology, University of Alberta, 5Department of Marketing, Business Economics, and Law, University of Alberta, 6Neuroscience Program, University of Illinois at Urbana-Champaign, 7Beckman Institute, University of Illinois at Urbana-Champaign

This article demonstrates an experimental design in which whole-body animated characters are used in conjunction with functional magnetic resonance imaging (fMRI) to investigate the neural correlates of observing virtual social interactions.

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Neuroscience

Brain Imaging Investigation of the Neural Correlates of Emotional Autobiographical Recollection
Ekaterina Denkova 1, Trisha Chakrabarty 1, Sanda Dolcos 1,2, Florin Dolcos 1,2,3,4
1Department of Psychiatry, University of Alberta, Edmonton, 2Psychology Department, University of Illinois, Urbana-Champaign, 3Neuroscience Program, University of Illinois, Urbana-Champaign, 4Beckman Institute for Advanced Science & Technology, University of Illinois, Urbana-Champaign

We present a protocol that allows investigation of the neural correlates of recollecting emotional autobiographical memories, using functional magnetic resonance imaging. This protocol can be used with both healthy and clinical participants.

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Neuroscience

Brain Imaging Investigation of the Neural Correlates of Emotion Regulation
Sanda Dolcos 1, Keen Sung 2, Ekaterina Denkova 3, Roger A. Dixon 4,5, Florin Dolcos 1,6,7
1Department of Psychology, University of Illinois, Urbana-Champaign, 2Department of Computing Science, University of Alberta, Edmonton, 3Department of Psychiatry, University of Alberta, Edmonton, 4Department of Psychology, University of Alberta, Edmonton, 5Centre for Neuroscience, University of Alberta, Edmonton, 6Neuroscience Program, University of Illinois, Urbana-Champaign, 7Beckman Institute, University of Illinois, Urbana-Champaign

We present a protocol that allows investigation of the neural correlates of deliberate and automatic emotion regulation, using functional magnetic resonance imaging. This protocol can be used in healthy participants, both young and older, as well as in clinical patients.

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Neuroscience

Brain Imaging Investigation of the Memory-Enhancing Effect of Emotion
Andrea Shafer 1, Alexandru Iordan 2, Roberto Cabeza 3, Florin Dolcos 1,4
1Centre for Neuroscience, University of Alberta, 2Neuroscience Program, University of Illinois, Urbana-Champaign, 3Center for Cognitive Neuroscience, Duke University, 4Psychology Department, Neuroscience Program, & Beckman Institute, University of Illinois, Urbana-Champaign

We present a protocol that uses functional magnetic resonance imaging to investigate the neural correlates of the memory-enhancing effect of emotion. This protocol allows identification of brain activity specifically linked to memory-related processing, contrary to more general perceptual processing, and can be used with healthy and clinical populations.

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Neuroscience

Brain Imaging Investigation of the Impairing Effect of Emotion on Cognition
Gloria Wong 1,2, Sanda Dolcos 1,3, Ekaterina Denkova 1, Rajendra Morey 4,5,6, Lihong Wang 4,5, Gregory McCarthy 6,7, Florin Dolcos 1,2,3,8,9
1Department of Psychiatry, University of Alberta, 2Centre for Neuroscience, University of Alberta, 3Department of Psychology, University of Illinois, 4Brain Imaging and Analysis Center, Duke University , 5Department of Psychiatry and Behavioral Sciences, Duke University , 6Mid-Atlantic Mental Illness Research Education and Clinical Center, VA Medical Center, 7Department of Psychology, Yale University, 8Neuroscience Program, University of Illinois, 9Beckman Institute for Advanced Science & Technology, University of Illinois

We present a protocol that allows investigation of the neural mechanisms mediating the detrimental impact of emotion on cognition, using functional magnetic resonance imaging. This protocol can be used with both healthy and clinical participants.

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Biology

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus
Mark Slabodnick 1,2, Bram Prevo 1,3, Peter Gross 1,4, Janet Sheung 1,5, Wallace Marshall 1,2
1Physiology Course, Marine Biological Laboratory, 2Department of Biochemistry & Biophysics, University of California San Francisco, 3Department of Physics and Astronomy, Vrije Universiteit Amsterdam, 4Max Planck Institute of Molecular Cell Biology and Genetics, 5Department of Physics, University of Illinois Urbana-Champaign

The giant ciliate Stentor coeruleus is a classical system for studying regeneration and wound healing in single cells. By imaging Stentor cells simultaneously at low and high magnification it is possible to measure cytoplasmic flows before, during, and after wounding.

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Neuroscience

A Comprehensive Protocol for Manual Segmentation of the Medial Temporal Lobe Structures
Matthew Moore *1, Yifan Hu *1, Sarah Woo 1, Dylan O'Hearn 1, Alexandru D. Iordan 2,3, Sanda Dolcos 1, Florin Dolcos 1,2,3
1Psychology Department, University of Illinois Urbana-Champaign, 2Neuroscience Program, University of Illinois Urbana-Champaign, 3Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign

The present work provides a comprehensive set of guidelines for manually tracing the medial temporal lobe (MTL) structures. This protocol can be applied to research involving structural and/or combined structural-functional magnetic resonance imaging (MRI) investigations of the MTL, in both healthy and clinical groups.

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Biology

Fluorescence Imaging with One-nanometer Accuracy (FIONA)
Yong Wang *1,2, En Cai *1,2, Janet Sheung 1,2, Sang Hak Lee 1,2, Kai Wen Teng 2,3, Paul R. Selvin 1,2,3
1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign

Single fluorophores can be localized with nanometer precision using FIONA. Here a summary of the FIONA technique is reported, and how to carry out FIONA experiments is described.

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Neuroscience

In Vivo Imaging of Dauer-specific Neuronal Remodeling in C. elegans
Nathan E. Schroeder 1, Kristen M. Flatt 1
1Department of Crop Sciences, University of Illinois Urbana-Champaign

Following exposure to specific environmental stressors, the nematode Caenorhabditis elegans undergoes extensive phenotypic plasticity to enter into a stress-resistant ‘dauer’ juvenile stage. We present methods for the controlled induction and imaging of neuroplasticity during dauer.

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Bioengineering

A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy
Samantha G. Knoll 1, M. Yakut Ali 1, M. Taher A. Saif 1
1Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign

Traditional techniques for fabricating polyacrylamide (PA) gels containing fluorescent probes involve sandwiching a gel between an adherent surface and a glass slide. Here, we show that coating this slide with poly-D-lysine (PDL) and fluorescent probes localizes the probes to within 1.6 µm from the gel surface.

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Neuroscience

Modification of a Colliculo-thalamocortical Mouse Brain Slice, Incorporating 3-D printing of Chamber Components and Multi-scale Optical Imaging
Bernard J. Slater 1, Anthony Y. Fan 2, Kevin A. Stebbings 1, M. Taher A. Saif 2, Daniel A. Llano 1,3
1Neuroscience Program, University of Illinois Urbana-Champaign, 2Mechanical Science and Engineering, University of Illinois Urbana-Champaign, 3Molecular and Integrative Physiology, University of Illinois Urbana-Champaign

Using multiple angles to cut the mouse pup brain, we improve upon a previously-described acute brain slice which captures the connections between most of the major auditory midbrain and forebrain structures.

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Bioengineering

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
Minyoung Kim 1,2, Hyunjoon Rhee 1,3, Ji Yoon Kang 1, Tae Song Kim 1, Rhokyun Kwak 1
1Center for BioMicrosystems, Korea Institute of Science and Technology, 2Department of Mechanical Engineering, Seoul National University, 3Department of Industrial Engineering, University of Illinois Urbana-Champaign

The protocol for a novel ion concentration polarization (ICP) platform that can stop the propagation of the ICP zone, regardless of the operating conditions is described. This unique ability of the platform lies in the use of merging ion depletion and enrichment, which are two polarities of the ICP phenomenon.

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Environment

Improving Infrared Spectroscopy Characterization of Soil Organic Matter with Spectral Subtractions
Andrew J. Margenot 1, Sanjai J. Parikh 2, Francisco J. Calderón 3
1Department of Crop Sciences, University of Illinois Urbana-Champaign, 2Department of Land, Air and Water Resources, University of California Davis, 3Central Great Plains Resources Management Research, USDA ARS

SOM underlies many soil functions and processes, but its characterization by FTIR spectroscopy is often challenged by mineral interferences. The described method can increase the utility of SOM analysis by FTIR spectroscopy by subtracting mineral interferences in soil spectra using empirically obtained mineral reference spectra.

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Chemistry

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
Kevin D. Clark 1,2, Stanislav S. Rubakhin 1,2, Jonathan V. Sweedler 1,2
1Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, 2Department of Chemistry, University of Illinois Urbana-Champaign

Post-transcriptional modifications of RNA represent an understudied layer of translation regulation that has recently been linked to central nervous system plasticity. Here, sample preparation and liquid chromatography-tandem mass spectrometry approach is described for simultaneous characterization of numerous RNA modifications in single neurons.

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Neuroscience

A Visuospatial Planning Task Coupled with Eye-Tracker and Electroencephalogram Systems
Marcos Domic-Siede 1, Martín Irani 2, Joaquín Valdés 3, María Rodríguez 4,5, Brice Follet 3,6, Marcela Perrone-Bertolotti 7,8, Tomás Ossandón 3
1Laboratorio de Neurociencia Cognitiva, Escuela de Psicología, Universidad Católica del Norte, 2Department of Psychology, University of Illinois Urbana-Champaign, 3Neurodynamics of Cognition Laboratory, Departamento de Psiquiatría, Pontificia Universidad Católica de Chile, 4Instituto de Ciencias de la Salud, Universidad de O’Higgins, 5Instituto de Ciencias de la Ingeniería, Universidad de O’Higgins, 6Department of Psychiatry, University Hospital of Saint-Etienne, 7Univ. Grenoble Alpes, Univ. Savoie Mont Blanc, CNRS, LPNC, 8Institut Universitaire de France (IUF)

The study of cognitive planning combining EEG and eye-tracking systems provides a multimodal approach to investigate the neural mechanisms that mediate cognitive control and goal-directed behavior in humans. Here, we describe a protocol for investigating the role of brain oscillations and eye movements in planning performance.

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Cancer Research

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity
Michael C. Lee *1,3,4, Sarah H. Gardner *2,3,4, Rodrigo Tapia Hernandez 1,3,4, Jefferson Chan 1,2,3,4
1Department of Chemistry, University of Illinois Urbana-Champaign, 2Department of Biochemistry, University of Illinois Urbana-Champaign, 3Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, 4Cancer Center of Illinois, University of Illinois Urbana-Champaign

Methods to measure ALDH1A1 activity in live cells are critical in cancer research due to its status as a biomarker of stemness. In this study, we employed an isoform-selective fluorogenic probe to determine the relative levels of ALDH1A1 activity in a panel of five ovarian cancer cell lines.

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Biology

Isolation and Characterization of the Murine Uterosacral Ligaments and Pelvic Floor Organs
Catalina S. Bastías 1, Lea M. Savard 2, Kevin N. Eckstein 2, Kathleen Connell 3, Callan M. Luetkemeyer 2,5, Virginia L. Ferguson 1,2,4, Sarah Calve 1,2,4
1Biomedical Engineering Program, University of Colorado Boulder, 2Paul M. Rady Department of Mechanical Engineering, University of Colorado Boulder, 3Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 4BioFrontiers Institute, University of Colorado Boulder, 5Department of Mechanical Science and Engineering, University of Illinois Urbana-Champaign

This article presents a detailed protocol for dissecting uterosacral ligaments and other pelvic floor tissues, including the cervix, rectum, and bladder in mice, to expand the study of female reproductive tissues.

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