JoVE Logo

Sign In

University Hospital Erlangen

6 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
Christina Stoeckle 1,2, Ioanna A. Rota 1, Eva Tolosa 3, Christoph Haller 4, Arthur Melms 5, Eleni Adamopoulou 1
1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen

This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.

image

Medicine

Surgical Fixation of Sternal Fractures: Preoperative Planning and a Safe Surgical Technique Using Locked Titanium Plates and Depth Limited Drilling
Stefan Schulz-Drost 1, Pascal Oppel 1, Sina Grupp 1, Sonja Schmitt 1, Roman Th. Carbon 2, Andreas Mauerer 3, Friedrich F. Hennig 1, Thomas Buder 4
1Orthopedic and Trauma Surgery, University Hospital Erlangen, 2Pediatric Surgery, University Hospital Erlangen, 3Orthopedic and Trauma Surgery, St.-Theresien Hospital, 4Institute of Anatomy I, University Erlangen-Nuremberg

Here we present a method to stabilize sternal fractures by using locked titanium plates in a low profile design. Performing subperiosteal dissection along the sternum while reducing the fracture, using depth limited drilling, and fixing the plates provides a safe surgical way.

image

Medicine

The Influence of Liver Resection on Intrahepatic Tumor Growth
Hannes H. Brandt 1, Valérie Nißler 2, Roland S. Croner 3
1Friedrich-Alexander-University Erlangen-Nürnberg (FAU), 2Department of Surgery, University Hospital Regensburg, 3Department of Surgery, University Hospital Erlangen

A high incidence of tumor recurrence after resection of liver metastases remains an unsolved problem. The illustrated mouse model may be useful to investigate the reasons for such recurrences. It combines a liver resection model with intrahepatic tumor cell injection for the first time.

image

Immunology and Infection

Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice
Vera Buchele 1,3, Maike Büttner-Herold 2, Tina Vogler 1,3, Markus F. Neurath 1,3, Kai Hildner 1,3
1Department of Internal Medicine 1, University Erlangen-Nürnberg, University Hospital Erlangen, 2Institute of Pathology, Department of Nephropathology, University Hospital Erlangen, 3Deutsches Zentrum Immuntherapie (DZI), University Hospital Erlangen

Here, we present a protocol that describes allogeneic hematopoietic stem cell transplantation and allows repetitive mini-endoscopic evaluations of the distal colon in situ for the presence, characteristics, and severity of colonic inflammation within live mice suffering from intestinal graft-versus-host disease.

image

Medicine

Cooling or Warming the Esophagus to Reduce Esophageal Injury During Left Atrial Ablation in the Treatment of Atrial Fibrillation
Jason Zagrodzky 1, Mark M. Gallagher 2, Lisa W. M. Leung 2, Tiffany Sharkoski 3, Pasquale Santangeli 4, Cory Tschabrunn 4, Jose M. Guerra 5, Bieito Campos 5, John MacGregor 6, Jamal Hayat 2, Brad Clark 7, Alex Mazur 8, Marcel Feher 9, Martin Arnold 9, Mark Metzl 10, Jose Nazari 10, Erik Kulstad 11
1St. David's South Austin Medical Center, 2St George's University Hospitals NHS Foundation Trust, St. George's, University of London, 3Hospital of the University of Pennsylvania, Perelman Center for Advanced Medicine, 4University of Pennsylvania Perelman School of Medicine, 5Hospital de la Santa Creu I Sant Pau, Universitat Autònoma de Barcelona, CIBERCV, 6PeaceHealth Medical Group, St. Joseph Medical Center, 7St. Vincent Hospital, 8University of Iowa, 9University Hospital Erlangen, 10NorthShore University Health System, 11Department of Emergency Medicine, University of Texas, Southwestern Medical Center

The goal of this protocol is to describe the use of esophageal temperature modulation to counteract esophageal thermal injury from left atrial ablation for the treatment of atrial fibrillation.

image

Biology

Generation of Patient-Derived Podocytes from Skin Biopsies
Victoria Rose 1, Janina Müller-Deile 1,2
1Department of Nephrology and Hypertension, University Hospital Erlangen, Friedrich-Alexander University (FAU) Erlangen-Nürnberg, 2Research Center on Rare Kidney Diseases (RECORD), University Hospital Erlangen

This manuscript describes a two-step protocol to generate patient-specific podocytes from dermal fibroblasts via episomal reprogramming into human-induced pluripotent stem cells (hiPSCs) and subsequent differentiation into podocytes.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved