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University of Naples "Federico II"

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Bone Marrow-derived Macrophage Production
Virginie Trouplin 1, Nicolas Boucherit 1, Laurent Gorvel 1, Filippo Conti 1, Giovanna Mottola 1,2, Eric Ghigo 1
1CNRS UMR 7278, IRD198, INSERM U1095, Aix-Marseille Université, 2Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II"

Macrophages have long been recognized as a critical component of the innate and adaptive immune responses. The recent explosion of knowledge pertaining to evolutionary, genetic, and biochemical aspects of the interaction between macrophages and microbes has renewed scientific attention to macrophages. This article describes a method to differentiate macrophages from mouse bone marrow.

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Immunology and Infection

Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens
Rachele Isticato 1, Ezio Ricca 1, Loredana Baccigalupi 2
1Department of Biology, Federico II University, 2Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II"

This protocol focuses on the use of bacterial spores as a "live" nanobiotechnological tool to adsorb heterologous molecules with various biological activities. The methods to measure the efficiency of adsorption are also shown.

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