INSERM U872

2 ARTICLES PUBLISHED IN JoVE

Biology

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

Miquella G. Chavez^1,2, Jimmy Hu¹, Kerstin Seidel¹, Chunying Li^1,3, Andrew Jheon¹, Adrien Naveau^4,5,6, Orapin Horst^1,7, Ophir D. Klein^1,8

¹Department of Orofacial Sciences and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, ²Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, ³Department of Pathology and Research Center, Zhongshan Hospital of Dalian University, ⁴Université Paris Descartes, Sorbonne Paris Cite, UMR S872, ⁵Centre de Recherche des Cordeliers, Université Pierre et Marie Curie, UMR S872, ⁶INSERM U872, ⁷Division of Endodontics, Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, ⁸Department of Pediatrics and Institute for Human Genetics, University of California, San Francisco

The continuously growing mouse incisor provides a model for studying renewal of dental tissues from dental epithelial stem cells (DESCs). A robust system for consistently and reliably obtaining these cells from the incisor and expanding them in vitro is reported here.

Medicine

Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

Jeremie Cosette^1,2,3, Rym Ben Abdelwahed², Sabrina Donnou-Triffault^1,4,5, Catherine Sautès-Fridman², Patrice Flaud^*3, Sylvain Fisson^*1,4,5

¹Genethon, ²Cordeliers Research Center, INSERM U872, ³Matter and Complex Systems Laboratory, Université Paris Diderot, ⁴INSERM U951, ⁵UMR_S951, Université d'Evry Val d'Essonne

Bioluminescence imaging is a well-known tool for localizing tumors and metastases, but quantification of these images often requires complex calculations and particular instruments. We describe the easy-to-use luminoscore method, based on precise acquisition conditions, requiring no calculations, and enabling tumor burden and treatment response to be monitored in mouse models.