Dr. William M Scholl College of Podiatric Medicine, Rosalind Franklin University of Medicine and Science

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Zebrafish Model of Diabetes Mellitus and Metabolic Memory

Robert V. Intine¹, Ansgar S. Olsen¹, Michael P. Sarras Jr.²

¹Dr. William M. Scholl College of Podiatric Medicine, Rosalind Franklin University of Medicine and Science, ²Chicago Medical School, Rosalind Franklin University of Medicine and Science

Metabolic memory is the phenomenon by which diabetic complications persist and progress unimpeded even after euglycemia is achieved pharmaceutically. Here we describe a diabetes mellitus zebrafish model which is unique in that it allows for the examination of the mitotically transmissible epigenetic components of metabolic memory in vivo.

Biology

An Assay for Lateral Line Regeneration in Adult Zebrafish

Gina C. Pisano^*1, Samantha M. Mason^*2, Nyembezi Dhliwayo¹, Robert V. Intine¹, Michael P. Sarras, Jr.²

¹Department of Biomedical Sciences, Dr. William M Scholl College of Podiatric Medicine, Rosalind Franklin University of Medicine and Science, ²Department of Cell Biology and Anatomy, Rosalind Franklin University of Medicine and Science

Because many zebrafish models of neurological and non-neurological diseases are studied in the adult fish rather than the embryo/larvae, we developed a quantitative lateral line regenerative assay that can be applied to adult zebrafish disease models. The assay involved resolution at the 1) neuromast and 2) individual hair cell levels.