Veterans Administration Greater Los Angeles Healthcare System

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina

Allison Sargoy¹, Steven Barnes^1,2,3, Nicholas C. Brecha^1,2,4, Luis Pérez De Sevilla Müller¹

¹Department of Neurobiology, University of California, Los Angeles, ²Veterans Administration Greater Los Angeles Healthcare System, ³Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences, Dalhousie University, ⁴Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine, University of California, Los Angeles

Immunohistochemistry protocols are used to study the localization of a specific protein in the retina. Calcium imaging techniques are employed to study calcium dynamics in retinal ganglion cells and their axons.

Biology

Specimen Collection and Analysis of the Duodenal Microbiome

Benjamin W. Dreskin¹, Kayti Luu¹, Tien S. Dong^1,2,3, Jihane Benhammou^1,2,4, Venu Lagishetty^2,3, John Vu¹, Daniel Sanford¹, Francisco Durazo^2,4, Vatche G. Agopian⁴, Jonathan P. Jacobs^1,2,3, Joseph R. Pisegna^1,5, Shehnaz K. Hussain⁶

¹Division of Gastroenterology, Hepatology and Parenteral Nutrition, Veterans Administration Greater Los Angeles Healthcare System, ²The Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at University of California, ³UCLA Microbiome Center, David Geffen School of Medicine at University of California, ⁴Department of Surgery, University of California, Los Angeles, ⁵Department of Medicine and Human Genetics, David Geffen School of Medicine at University of California, ⁶Cedars-Sinai Cancer and Department of Medicine, Cedars-Sinai Medical Center

In this manuscript, we discuss a novel method to sample and analyze the duodenal microbiome. This method provides an accurate depiction of microbial diversity and composition in the duodenum and could be useful for further investigation of the duodenal microbiome.