JoVE Logo
Faculty Resource Center

Sign In

Toronto General Hospital

9 ARTICLES PUBLISHED IN JoVE

image

Medicine

Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
Sharif U. Ahmed 1, Murtuza Zair 1, Kui Chen 1, Matthew Iu 1, Feng He 1, Oyedele Adeyi 2, Sean P. Cleary 3, Anand Ghanekar 1,3
1Toronto General Research Institute, University Health Network, 2Department of Pathology, University Health Network, 3Division of General Surgery, University Health Network

Human tumor xenografts in immunodeficient mice are valuable tools to study cancer biology. Specific protocols to generate subcutaneous and intrahepatic xenografts from human hepatocellular carcinoma cells or tumor fragments are described. Liver regeneration induced by partial hepatectomy in recipient mice is presented as a strategy to facilitate intrahepatic engraftment.

image

Medicine

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts
Jan M. Knaak *1,2, Vinzent N. Spetzler *1,2, Nicolas Goldaracena 1,2, Kristine S. Louis 1,2, Nazia Selzner 1,3, Markus Selzner 1,2
1Multi Organ Transplant Program, Toronto General Hospital, 2Department of Surgery, Toronto General Hospital, 3Department of Medicine, Toronto General Hospital

Marginal grafts, such as fatty livers, grafts from older donors, or livers retrieved after cardiac death (DCD) tolerate conventional, cold static storage only poorly. We developed a novel model of subnormothermic ex vivo liver perfusion for preservation, assessment, and repair of marginal liver grafts prior to transplantation.

image

Medicine

Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt
Vinzent N. Spetzler 1, Nicolas Goldaracena 1, Jan M. Knaak 1, Kristine S. Louis 1, Nazia Selzner 1, Markus Selzner 1
1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital

Experimental animal research plays a pivotal role in the development of clinical transplantation practice. The porcine orthotopic liver transplantation model (OLTx) closely resembles human conditions and is frequently used in clinically oriented research. The following protocol contains all information for a reliable porcine OLTx model using an active porto-caval-jugular shunt.

image

Medicine

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
J. Moritz Kaths 1,2, Vinzent N. Spetzler 1, Nicolas Goldaracena 1, Juan Echeverri 1, Kristine S. Louis 1, Daniel B. Foltys 3, Mari Strempel 4, Paul Yip 5, Rohan John 5, Istvan Mucsi 1, Anand Ghanekar 1, Darius Bagli 6,7, Lisa Robinson 2, Markus Selzner 1
1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, Toronto, 3Department of General, Visceral & Transplant Surgery, University Medical Center Mainz, 4Department of Abdominal, Vascular & Transplant Surgery, Merheim Medical Center Cologne, 5Laboratory Medicine & Pathobiology, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, The Hospital for Sick Children, Toronto, 7Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto

The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to  transplantation.

image

Medicine

Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol
J. Moritz Kaths 1,2, Juan Echeverri 1,3, Nicolas Goldaracena 1, Kristine S. Louis 1, Paul Yip 4, Rohan John 4, Istvan Mucsi 5, Anand Ghanekar 1, Darius Bagli 6, Markus Selzner 1, Lisa A. Robinson 2
1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, 3Programa de Doctorat en Medicina, La Universitat Autónoma de Barcelona, 4Laboratory Medicine and Pathobiology, Toronto General Hospital, 5Department of Medicine, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, Developmental & Stem Cell Biology, The Hospital for Sick Children

Porcine models of organ transplantation provide an important platform to study mechanisms of organ preservation. This article describes a heterotopic porcine renal autotransplantation model, which allows investigating new approaches to improve the outcome of transplantation using marginal kidney grafts.

image

Medicine

Using a Chemical Biopsy for Graft Quality Assessment
Iga Stryjak *1, Natalia Warmuzińska *1, Kamil Łuczykowski 1, Matyas Hamar 2, Peter Urbanellis 2, Emilia Wojtal 3, Marek Masztalerz 3, Markus Selzner 2,4, Zbigniew Włodarczyk 3, Barbara Bojko 1
1Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, 2Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, University Health Network, 3Department of Transplantology and General Surgery, Collegium Medicum in Bydgoszcz, Antoni Jurasz University Hospital No. 1 in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland, 4Department of Medicine, Toronto General Hospital

The protocol presents utilization of the chemical biopsy approach followed by comprehensive metabolomic and lipidomic analysis for quality assessment of kidney grafts allocated for transplantation.

image

Medicine

Normothermic Ex Vivo Pancreas Perfusion for the Preservation of Pancreas Allografts before Transplantation
Catherine Parmentier 1, Samrat Ray 1, Laura Mazilescu 1,2, Masataka Kawamura 1, Yuki Noguchi 1, Emmanuel Nogueira 1, Sujani Ganesh 1, Bhranavi Arulratnam 1, Sangeetha Kalimuthu 3, Markus Selzner 1, Trevor Reichman 1
1Ajmera Transplant Program, University Health Network, Toronto General Hospital, 2Department of General, Visceral, and Transplantation Surgery, University Hospital Essen, 3Department of Pathology, University Health Network, Toronto General Hospital

Normothermic ex vivo machine perfusion (NEVP) has scarcely been explored for the preservation of pancreas allografts. We present an innovative preservation technique for pancreas allografts before transplantation.

image

Medicine

Large-Animal Model of Donation after Circulatory Death and Normothermic Regional Perfusion for Cardiac Assessment
Khalil Khalil *1,2, Roberto V. P. Ribeiro *3, Julgans S. Alvarez 4,5, Mitesh V. Badiwala 4,5, Shant Der Sarkissian 1,2, Nicolas Noiseux 1,2
1Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), 2Department of Surgery, Faculty of Medicine, Université de Montréal, 3Division of Cardiac Surgery, Dalhousie University, 4Division of Cardiovascular Surgery, Peter Munk Cardiac Centre, Toronto General Hospital, 5Department of Surgery, University of Toronto

The protocol describes a large-animal (porcine) model of donation after circulatory death, followed by thoracoabdominal normothermic regional perfusion that closely simulates the clinical scenario in heart transplantation, and has the potential to facilitate therapeutic studies and strategies.

image

Medicine

Surgical Tips and Tricks for Performing Porcine Pancreas Transplantation
Samrat Ray 1, Catherine Parmentier 1, Laura Mazilescu 1,2, Masataka Kawamura 1, Yuki Noguchi 1, Emmanuel Nogueira 1, Sujani Ganesh 1, Bhranavi Arulratnam 1, Markus Selzner 1, Trevor Reichman 1
1Ajmera Transplant Centre, University Health Network, Toronto general hospital, 2Department of general, visceral, and transplantation surgery, University Hospital Essen

The video article summarizes the technique of pancreatectomy and pancreas allotransplantation in a porcine 3-day survival model with a step-by-step description of the method and emphasis on the surgical tips and tricks to deal with the precarious and delicate porcine visceral anatomy.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved